BBa_K571003 1 BBa_K571003 acsCD/pSB1C3 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z *organism: Gluconacetobacter hansenii ATCC 23769 *GenBank: AB091060.1 This biobrick contains the remaining 2 cellulose synthase enzyme complex, acsC and acsD of 4,363 bp in total (full CDS), again containing Xba I and Spe I restriction sites cloned in pSB1C3. The acsC gene is the main composite in the formation of the membrane complex of cellulose synthase and proposed to be involved in the export of the polymer across the bacterial cell wall. The acsD gene is involved in the crystallization of the mature cellulose by cleaves the intrastrand ??-1,4 linkages in the cellulose chain and is proposed to have a role in the release of the growing polymer from the cell. false true _739_ 0 10422 9 It's complicated false acsCD is long base pairs gene, so more than one primer is used during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results. false Lu-Chu Ke annotation2150411 1 acsCD range2150411 1 1 4363 BBa_K571004 1 BBa_K571004 R0011+RBS/pSB1C3 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z BBa_R0011 This biobrick contains the BBa_R0011 promoter of 80 bp cloned in pSB1C3 with EcoRI and XbaI restriction site which will can be induced by IPTG. RBS is added to the end of the sequence in order to enable gene expression to perform well. false false _739_ 0 10422 9 It's complicated false none. false Lu-Chu Ke annotation2151464 1 R0011 range2151464 1 1 55 annotation2151465 1 RBS range2151465 1 64 80 BBa_K571006 1 BBa_K571006 R0011+acsCD/pSB1C3 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z *acsCD: GenBank AB091060.1 *promoter: iGEM registry of standard biological part The biobrick is composite element, it is the combination of BBa_K571001 and BBa_K571003. The promoter binding to the upstream of acsCD gene, IPTG as the inducer, activates the acsCD gene to synthesize protein. The sequence of acsCD and promoter is amplified by PCR, followed by enzyme digestion and ligation to bind the 2 sequences with the backbone-pSB1C3. The order of restriction enzyme is EcoRI, XbaI and SpeI. false false _739_ 0 10422 9 It's complicated false It is a very long sequence, therefore below is a few measures to take note during cloning. *The concentration agarose gel during gel electrophoresis should be about 0.8-1% *The enzyme digestion reaction time is advised to be elongated so that digestion could be more perfect. *The respective restriction enzyme at the both end of primer should not cut the sequence of another gene. false Lu-Chu Ke component2151361 1 BBa_K571004 component2151363 1 BBa_K571003 annotation2151361 1 BBa_K571004 range2151361 1 1 80 annotation2151363 1 BBa_K571003 range2151363 1 87 4449 BBa_K571003_sequence 1 atgcttcgtccctgtccgccggtcttctcgcaacgacctgcgtcgcaggtctgttgctgcaggcgaacggcgcacgggcacagcaggcggcagaggcgcaggccccggccagcagcaccaccatgatgcaggcagctaccgttgcccccgcccagagtgggcaggccgcggtggtgcagcggctggtgcagcaggcccgtttctggatgcagcagcaccagtatgaaaatgcgcgccagtccctgcagagtgctgcgcgacttgcaccggattccgttgatctgctggaggccgagggcgaatatcagtcgcatatcggcaatcgtgatgccgcccttgatacgcagcgtcgcctgcatcaggccgcaccaggcagcacgtatgaaagccagttgaacgacctgctgcatgaacaggcgatttcccagccggaccttgcgcatgcgcgctcgcttgccgcatccgggcacagcgatcaggcggtggaagcgtaccagcacctgttcaacggttcgacgcccacgccctcgctcgcggttgaatattaccagacgctggctgacgtctcggggcaggccggcacggcacaggatgggctgatccgtctggtcaaggccaatccttccgatttccgggcgcaactggcccttgcgcaggtcctgacctatcagcccggcacccggatggaggggctgcagcggcttcaggcgctccagaagtaccagtcttccgccccggtggaggctgcgacggcggaaaaatcatatcgccagacgctgtcatggttaccggttacacccgaaacactgccgttgatgcagaaatggctggacgcacatccatccgacagcgcattgcggacccatatggcagaaccggcaggcgggccgccggataaaggcgcgttggcgcggcaggacgggttcaaggcgttgaacgccggacgtctgtccgcagcccaggccgcgttccagagcgcactgaacctgaatgccaaggacggcgatgccctgggcggccttggccttgtcgccatgcgcgcgggccataacgaggaagcgcatcgttacctcgaagacgcgatcgcggctgatcccaagaatgcggcgcactggcgtccggcactggctggcatggccgtgggcgaggaatatggcagcgtccgtcgcctgatcgccagtggacagacacaggaagccgaacagcgcctgatgacgctggcgcgtcagcccggacagtccgagggcgcgacccttatgctggcggatttgcagcgcagcacgggccagaccggcgaggccgagcgcaattaccgggcgatcctggcgcgcaatggtgacaatcccatcgcactgatgggactggcccgtgtgctgatgggggaaggccaggagaacgaagcgaacgccctgctgtcgcgccttggtggtcgctatagcgatcaggtgcagcagatcgaggtttcgggtatcatggccgaagctgcccgcacgtcggattcagcgcagaaggtcagccttctgcggcaggccatgaccaaggccccggatgacccgtggttgcgcatcaaccttgccaatgcgctgcaacagcaaggtgacagcgcggaagcggccaatgtcatgcgtccgcttctgaccagcccgcgtacgccggccgattatcaggcggcgatcctgtatgcgtccggcaatggcaatgatacgctggcacgccgtttgcttgcgggactgtcgccggatgactattccccggccatccgcaccattgccgatgaaatggcgatcaaggccgatctggccagccgcctttcgatggtttcgaatcccacgccgcttgtgcgcgaggctctggcggcgccggacccgacgggcgcgcgtggtgtggcggtggccgacctgttccggcagcgtggtgacatgctgcatgcgcatatggcgctgcgcatcgcctccacgcgcaatattgacctgacgaccgaacagcggctggcctatgccaccgaatatatgaagatcagcaatcccgttgccgcagcgcgcctgcttgcaccgttgggcgatggtagtggaaccgcaaccggttccgcgatgtcgccggatcagcggcagacgctgatgcagttgcgcatgggcatttcggtggcacagtcggacctgctgaaccagcgtggcgatcaggcggcggcgtgtgaccaccttgcgcccgcgttgcaggccgatccagaggcgacatcccccaagcttgcgttggcgcgactgtataacgggcgtggcaagtatgggcacgcgctggacatcgaccttgctgttcttcggcataatccgcaggaccttgatgcgcggcaggccgccgtgcaggccgcggcgaatgacggcaaggacaacctggccatgcaactggcgcaagatggggtccagcagtcgccgatggatgcgcgcagctggcttggcatggcggtggcggaccacgctgtgggccatggcgaccggacgcttgctgacctgcgccgggcgtatgaattgcgcctgcagcagctcaagatcagtcggggtgatgccatcggcggtgatgaaacgcaggcgaccgcgccgccgacggccaatccgttccgtcgcgatgcatacgggcatgcgctgtcgctgggtgcgcccccgggtgagaacgggtacagcacggcaggaagcgtgcccgagatttcggaccagatgctgtcctccatcaacgggcagatccataccctgtccgaagacatggcgccgtctgttgatgccggccttggcttccgtgtccgttccggcaccccgggcatgggcgcgttgaccgaggcatccgtgccgatcgtcgggcgcatcccgctgcaggctggtacttcggcgctgacattcacggccacgccaaccttcctgacgtcggggcacctgccgcagaccgggtatgatataccgcgtttcggcaccaacctgttcgcgctggaacggaacctgcagaaccagaacaacagcgcggaacatcgcatcaataccgatacgatcgggcgcgaggcaggtgtggcgcctgatgtccgctttgccaataactgggtcagtgccgatgtcggtgcctcgcctctgggctttacgctgccgaacgtgatcggtggtgttgaattcgccccgcgtgtcgggcctgtgaccttccgtgtcagtggggaacgccgttccattaccaacagcgtcctgtcctatggcgggatgaccgatgccctgaccggcaagaagtggggcggtgttgtcaccaaccacttccatggacaggtcgaggctacgctgggcaataccattgtgtatggtggtggtggttatgccatccagaccggccatcatgtccagagcaataccgaagtggaaggcgggctgggggcgaataccctggtttatcgcaaccgcaagcacgaagtgcgtgtcggcgtgaacctgacctatctcggctataagcataacgaggacttctatacttacgggcagggtggctacttctcgccgcagtcctattttgcggcaacggtgcccgtccggtattccgggcatagtggcctgtttgactgggatgtcaccgggtccatcgggtatcagttgtttcatgaacacagttcggccttcttcccgaccaatcctgtgtatcaggcccttgcgaacgggctggcgggtgtatctactgctgaactatccttggaatcggccaggtatcccggcgatgatgtcggtagccttgttggtggtttcgatggcagggtgggttatcgcgtcagccattcgctgcgccttgatctgtccggtcgtttccagaaggctggaaactgggatgagggcggcgccatgatctcggctcactatcttattatggaccagtaatgacaatttttgagaaaaaaccggatttcaccctgtttcttcagaccctgtcatgggaaattgatgatcaggtcgggatcgaggtcaggaacgagctcctgcgtgaggtcggacggggcatgggcacgcgcatcatgccgccgccgtgccagaccgtggacaagctgcagatcgaactgaacgcgcttctggccctgatcggctggggcaccgtgacgctcgaactcctcagcgaggaccagtccctgcgcatcgtgcatgaaaacctgccgcaggttggcagcgcgggcgaaccttcgggcacgtggctggctccggtgctggaggggctgtatggccgctgggtgacgtcgcaggcgggtgcgtttggtgattatgtcgttacacgcgatgtggacgccgaggatctgaatgctgttccgcgtcagaccatcatcatgtacatgcgcgtgcgcagttccgcgacctga BBa_K571006_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagttaaggaggtaaaaaaatactagatgcttcgtccctgtccgccggtcttctcgcaacgacctgcgtcgcaggtctgttgctgcaggcgaacggcgcacgggcacagcaggcggcagaggcgcaggccccggccagcagcaccaccatgatgcaggcagctaccgttgcccccgcccagagtgggcaggccgcggtggtgcagcggctggtgcagcaggcccgtttctggatgcagcagcaccagtatgaaaatgcgcgccagtccctgcagagtgctgcgcgacttgcaccggattccgttgatctgctggaggccgagggcgaatatcagtcgcatatcggcaatcgtgatgccgcccttgatacgcagcgtcgcctgcatcaggccgcaccaggcagcacgtatgaaagccagttgaacgacctgctgcatgaacaggcgatttcccagccggaccttgcgcatgcgcgctcgcttgccgcatccgggcacagcgatcaggcggtggaagcgtaccagcacctgttcaacggttcgacgcccacgccctcgctcgcggttgaatattaccagacgctggctgacgtctcggggcaggccggcacggcacaggatgggctgatccgtctggtcaaggccaatccttccgatttccgggcgcaactggcccttgcgcaggtcctgacctatcagcccggcacccggatggaggggctgcagcggcttcaggcgctccagaagtaccagtcttccgccccggtggaggctgcgacggcggaaaaatcatatcgccagacgctgtcatggttaccggttacacccgaaacactgccgttgatgcagaaatggctggacgcacatccatccgacagcgcattgcggacccatatggcagaaccggcaggcgggccgccggataaaggcgcgttggcgcggcaggacgggttcaaggcgttgaacgccggacgtctgtccgcagcccaggccgcgttccagagcgcactgaacctgaatgccaaggacggcgatgccctgggcggccttggccttgtcgccatgcgcgcgggccataacgaggaagcgcatcgttacctcgaagacgcgatcgcggctgatcccaagaatgcggcgcactggcgtccggcactggctggcatggccgtgggcgaggaatatggcagcgtccgtcgcctgatcgccagtggacagacacaggaagccgaacagcgcctgatgacgctggcgcgtcagcccggacagtccgagggcgcgacccttatgctggcggatttgcagcgcagcacgggccagaccggcgaggccgagcgcaattaccgggcgatcctggcgcgcaatggtgacaatcccatcgcactgatgggactggcccgtgtgctgatgggggaaggccaggagaacgaagcgaacgccctgctgtcgcgccttggtggtcgctatagcgatcaggtgcagcagatcgaggtttcgggtatcatggccgaagctgcccgcacgtcggattcagcgcagaaggtcagccttctgcggcaggccatgaccaaggccccggatgacccgtggttgcgcatcaaccttgccaatgcgctgcaacagcaaggtgacagcgcggaagcggccaatgtcatgcgtccgcttctgaccagcccgcgtacgccggccgattatcaggcggcgatcctgtatgcgtccggcaatggcaatgatacgctggcacgccgtttgcttgcgggactgtcgccggatgactattccccggccatccgcaccattgccgatgaaatggcgatcaaggccgatctggccagccgcctttcgatggtttcgaatcccacgccgcttgtgcgcgaggctctggcggcgccggacccgacgggcgcgcgtggtgtggcggtggccgacctgttccggcagcgtggtgacatgctgcatgcgcatatggcgctgcgcatcgcctccacgcgcaatattgacctgacgaccgaacagcggctggcctatgccaccgaatatatgaagatcagcaatcccgttgccgcagcgcgcctgcttgcaccgttgggcgatggtagtggaaccgcaaccggttccgcgatgtcgccggatcagcggcagacgctgatgcagttgcgcatgggcatttcggtggcacagtcggacctgctgaaccagcgtggcgatcaggcggcggcgtgtgaccaccttgcgcccgcgttgcaggccgatccagaggcgacatcccccaagcttgcgttggcgcgactgtataacgggcgtggcaagtatgggcacgcgctggacatcgaccttgctgttcttcggcataatccgcaggaccttgatgcgcggcaggccgccgtgcaggccgcggcgaatgacggcaaggacaacctggccatgcaactggcgcaagatggggtccagcagtcgccgatggatgcgcgcagctggcttggcatggcggtggcggaccacgctgtgggccatggcgaccggacgcttgctgacctgcgccgggcgtatgaattgcgcctgcagcagctcaagatcagtcggggtgatgccatcggcggtgatgaaacgcaggcgaccgcgccgccgacggccaatccgttccgtcgcgatgcatacgggcatgcgctgtcgctgggtgcgcccccgggtgagaacgggtacagcacggcaggaagcgtgcccgagatttcggaccagatgctgtcctccatcaacgggcagatccataccctgtccgaagacatggcgccgtctgttgatgccggccttggcttccgtgtccgttccggcaccccgggcatgggcgcgttgaccgaggcatccgtgccgatcgtcgggcgcatcccgctgcaggctggtacttcggcgctgacattcacggccacgccaaccttcctgacgtcggggcacctgccgcagaccgggtatgatataccgcgtttcggcaccaacctgttcgcgctggaacggaacctgcagaaccagaacaacagcgcggaacatcgcatcaataccgatacgatcgggcgcgaggcaggtgtggcgcctgatgtccgctttgccaataactgggtcagtgccgatgtcggtgcctcgcctctgggctttacgctgccgaacgtgatcggtggtgttgaattcgccccgcgtgtcgggcctgtgaccttccgtgtcagtggggaacgccgttccattaccaacagcgtcctgtcctatggcgggatgaccgatgccctgaccggcaagaagtggggcggtgttgtcaccaaccacttccatggacaggtcgaggctacgctgggcaataccattgtgtatggtggtggtggttatgccatccagaccggccatcatgtccagagcaataccgaagtggaaggcgggctgggggcgaataccctggtttatcgcaaccgcaagcacgaagtgcgtgtcggcgtgaacctgacctatctcggctataagcataacgaggacttctatacttacgggcagggtggctacttctcgccgcagtcctattttgcggcaacggtgcccgtccggtattccgggcatagtggcctgtttgactgggatgtcaccgggtccatcgggtatcagttgtttcatgaacacagttcggccttcttcccgaccaatcctgtgtatcaggcccttgcgaacgggctggcgggtgtatctactgctgaactatccttggaatcggccaggtatcccggcgatgatgtcggtagccttgttggtggtttcgatggcagggtgggttatcgcgtcagccattcgctgcgccttgatctgtccggtcgtttccagaaggctggaaactgggatgagggcggcgccatgatctcggctcactatcttattatggaccagtaatgacaatttttgagaaaaaaccggatttcaccctgtttcttcagaccctgtcatgggaaattgatgatcaggtcgggatcgaggtcaggaacgagctcctgcgtgaggtcggacggggcatgggcacgcgcatcatgccgccgccgtgccagaccgtggacaagctgcagatcgaactgaacgcgcttctggccctgatcggctggggcaccgtgacgctcgaactcctcagcgaggaccagtccctgcgcatcgtgcatgaaaacctgccgcaggttggcagcgcgggcgaaccttcgggcacgtggctggctccggtgctggaggggctgtatggccgctgggtgacgtcgcaggcgggtgcgtttggtgattatgtcgttacacgcgatgtggacgccgaggatctgaatgctgttccgcgtcagaccatcatcatgtacatgcgcgtgcgcagttccgcgacctga BBa_K571004_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagttaaggaggtaaaaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z