BBa_K571003 1 BBa_K571003 acsCD/pSB1C3 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z *organism: Gluconacetobacter hansenii ATCC 23769 *GenBank: AB091060.1 This biobrick contains the remaining 2 cellulose synthase enzyme complex, acsC and acsD of 4,363 bp in total (full CDS), again containing Xba I and Spe I restriction sites cloned in pSB1C3. The acsC gene is the main composite in the formation of the membrane complex of cellulose synthase and proposed to be involved in the export of the polymer across the bacterial cell wall. The acsD gene is involved in the crystallization of the mature cellulose by cleaves the intrastrand ??-1,4 linkages in the cellulose chain and is proposed to have a role in the release of the growing polymer from the cell. false true _739_ 0 10422 9 It's complicated false acsCD is long base pairs gene, so more than one primer is used during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results. false Lu-Chu Ke annotation2150411 1 acsCD range2150411 1 1 4363 BBa_K571004 1 BBa_K571004 R0011+RBS/pSB1C3 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z BBa_R0011 This biobrick contains the BBa_R0011 promoter of 80 bp cloned in pSB1C3 with EcoRI and XbaI restriction site which will can be induced by IPTG. RBS is added to the end of the sequence in order to enable gene expression to perform well. false false _739_ 0 10422 9 It's complicated false none. false Lu-Chu Ke annotation2151464 1 R0011 range2151464 1 1 55 annotation2151465 1 RBS range2151465 1 64 80 BBa_K571000 1 BBa_K571000 acsAB/pSB1C3 2011-10-01T11:00:00Z 2015-05-08T01:12:44Z *organism: Gluconacetobacter hansenii ATCC 23769 *GenBank: AB091060.1 The acs operon in Gluconacetobacter hansenii is the mechanism that synthesize cellulose synthase, acsAB is one of the gene sequence. The acsAB will produce protein with 2 subunits; one is catalytic subunit which is able to transform UDP-glucose to UDP, another one would be the regulatory subunit of the cellulose synthase which will bind with cyclic di-GMP and activated. From the primer we design, the acsAB sequence is amplified from PCR from the genomic DNA of Gluconacetobacter hansenii, XbaI and SpeI is also added at the both end of primer as the cutting site, so that acsAB and pSB1C3 could easily bind to it. false false _739_ 0 10422 9 It's complicated false By comparative, acsAB is long base pairs gene, so we need more primer during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results. false Lu-Chu Ke annotation2149953 1 acsAB range2149953 1 1 4653 BBa_K571002 1 BBa_K571002 CMCax+Ccp/pSB1C3 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z *organism: Gluconacetobacter hansenii ATCC 23769 *GenBank: EFG85213.1, AAA16970.1 The biobrick contains the cellulose synthesis related genes situated at the upstream and downstream of the cellulose synthase operon, which have been proposed to increase the production of bacteria cellulose, altough their function has not been completely elucidated. This biobrick contains the cmcax and ccp genes in 2,092 bp containing SpeI and PstI restriction sites cloned in pSB1C3. The cmcax (endo-1,4-β-glucanase) is required to enhance the cellulose biosynthesis by providing carbon source to acs operon whereas the ccp encoding the cellulose complementing protein. false false _739_ 0 10422 9 It's complicated false The cmcax gene has 2 Pst I cutting site while ccp gene has a EcoRI cutting site. During ligation, Spe and AlwN I is used to do enzyme digestion to get the cmcax sequence (approx. 1kb), followed by the binding of pSB1C3. Next, AlwNI and PstI will be used to do the second enzyme digestion in order to get ccp sequence (approx. 1kb), followed by the binding of pSB1C3. It is necessary to avoid restriction of wanted DNA while intentionally cutting the ends of DNA. false Lu-Chu Ke annotation2150405 1 Ccp range2150405 1 1030 2092 annotation2150404 1 CMCax range2150404 1 1 1029 BBa_K571007 1 BBa_K571007 R0011+acsAB+acsCD+CMCax+Ccp/pSB1C3 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z *organism: Gluconacetobacter hansenii ATCC 23769 *acs operon: GenBank AB091060.1 *CMCax: GenBank EFG85213.1 *Ccp: GenBank AAA16970. The biobrick consists of complete acs operon gene with CMCax and Ccp gene and a promoter which will activate the operon when it is induced by IPTG, cellulose synthase will be synthesized, which later use to produce cellulose. false false _739_ 0 10422 9 Not in stock true 1.The sequence has a few restriction enzyme cutting sites, therefore checking is needed when we do enzyme digestion and ligation so that the sequence is correctly connected. 2.It is a very long sequence, below is few measures to take note during cloning. *The concentration agarose gel during gel electrophoresis should be about 0.8-1% *The enzyme digestion reaction time is advised to be elongated so that digestion could be more perfect. *The respective restriction enzyme at the both end of primer should not cut the sequence of another gene false Lu-Chu Ke component2151387 1 BBa_K571004 component2151394 1 BBa_K571002 component2151389 1 BBa_K571000 component2151391 1 BBa_K571003 annotation2151394 1 BBa_K571002 range2151394 1 9115 11206 annotation2151387 1 BBa_K571004 range2151387 1 1 80 annotation2151391 1 BBa_K571003 range2151391 1 4746 9108 annotation2151389 1 BBa_K571000 range2151389 1 87 4739 BBa_K571003_sequence 1 atgcttcgtccctgtccgccggtcttctcgcaacgacctgcgtcgcaggtctgttgctgcaggcgaacggcgcacgggcacagcaggcggcagaggcgcaggccccggccagcagcaccaccatgatgcaggcagctaccgttgcccccgcccagagtgggcaggccgcggtggtgcagcggctggtgcagcaggcccgtttctggatgcagcagcaccagtatgaaaatgcgcgccagtccctgcagagtgctgcgcgacttgcaccggattccgttgatctgctggaggccgagggcgaatatcagtcgcatatcggcaatcgtgatgccgcccttgatacgcagcgtcgcctgcatcaggccgcaccaggcagcacgtatgaaagccagttgaacgacctgctgcatgaacaggcgatttcccagccggaccttgcgcatgcgcgctcgcttgccgcatccgggcacagcgatcaggcggtggaagcgtaccagcacctgttcaacggttcgacgcccacgccctcgctcgcggttgaatattaccagacgctggctgacgtctcggggcaggccggcacggcacaggatgggctgatccgtctggtcaaggccaatccttccgatttccgggcgcaactggcccttgcgcaggtcctgacctatcagcccggcacccggatggaggggctgcagcggcttcaggcgctccagaagtaccagtcttccgccccggtggaggctgcgacggcggaaaaatcatatcgccagacgctgtcatggttaccggttacacccgaaacactgccgttgatgcagaaatggctggacgcacatccatccgacagcgcattgcggacccatatggcagaaccggcaggcgggccgccggataaaggcgcgttggcgcggcaggacgggttcaaggcgttgaacgccggacgtctgtccgcagcccaggccgcgttccagagcgcactgaacctgaatgccaaggacggcgatgccctgggcggccttggccttgtcgccatgcgcgcgggccataacgaggaagcgcatcgttacctcgaagacgcgatcgcggctgatcccaagaatgcggcgcactggcgtccggcactggctggcatggccgtgggcgaggaatatggcagcgtccgtcgcctgatcgccagtggacagacacaggaagccgaacagcgcctgatgacgctggcgcgtcagcccggacagtccgagggcgcgacccttatgctggcggatttgcagcgcagcacgggccagaccggcgaggccgagcgcaattaccgggcgatcctggcgcgcaatggtgacaatcccatcgcactgatgggactggcccgtgtgctgatgggggaaggccaggagaacgaagcgaacgccctgctgtcgcgccttggtggtcgctatagcgatcaggtgcagcagatcgaggtttcgggtatcatggccgaagctgcccgcacgtcggattcagcgcagaaggtcagccttctgcggcaggccatgaccaaggccccggatgacccgtggttgcgcatcaaccttgccaatgcgctgcaacagcaaggtgacagcgcggaagcggccaatgtcatgcgtccgcttctgaccagcccgcgtacgccggccgattatcaggcggcgatcctgtatgcgtccggcaatggcaatgatacgctggcacgccgtttgcttgcgggactgtcgccggatgactattccccggccatccgcaccattgccgatgaaatggcgatcaaggccgatctggccagccgcctttcgatggtttcgaatcccacgccgcttgtgcgcgaggctctggcggcgccggacccgacgggcgcgcgtggtgtggcggtggccgacctgttccggcagcgtggtgacatgctgcatgcgcatatggcgctgcgcatcgcctccacgcgcaatattgacctgacgaccgaacagcggctggcctatgccaccgaatatatgaagatcagcaatcccgttgccgcagcgcgcctgcttgcaccgttgggcgatggtagtggaaccgcaaccggttccgcgatgtcgccggatcagcggcagacgctgatgcagttgcgcatgggcatttcggtggcacagtcggacctgctgaaccagcgtggcgatcaggcggcggcgtgtgaccaccttgcgcccgcgttgcaggccgatccagaggcgacatcccccaagcttgcgttggcgcgactgtataacgggcgtggcaagtatgggcacgcgctggacatcgaccttgctgttcttcggcataatccgcaggaccttgatgcgcggcaggccgccgtgcaggccgcggcgaatgacggcaaggacaacctggccatgcaactggcgcaagatggggtccagcagtcgccgatggatgcgcgcagctggcttggcatggcggtggcggaccacgctgtgggccatggcgaccggacgcttgctgacctgcgccgggcgtatgaattgcgcctgcagcagctcaagatcagtcggggtgatgccatcggcggtgatgaaacgcaggcgaccgcgccgccgacggccaatccgttccgtcgcgatgcatacgggcatgcgctgtcgctgggtgcgcccccgggtgagaacgggtacagcacggcaggaagcgtgcccgagatttcggaccagatgctgtcctccatcaacgggcagatccataccctgtccgaagacatggcgccgtctgttgatgccggccttggcttccgtgtccgttccggcaccccgggcatgggcgcgttgaccgaggcatccgtgccgatcgtcgggcgcatcccgctgcaggctggtacttcggcgctgacattcacggccacgccaaccttcctgacgtcggggcacctgccgcagaccgggtatgatataccgcgtttcggcaccaacctgttcgcgctggaacggaacctgcagaaccagaacaacagcgcggaacatcgcatcaataccgatacgatcgggcgcgaggcaggtgtggcgcctgatgtccgctttgccaataactgggtcagtgccgatgtcggtgcctcgcctctgggctttacgctgccgaacgtgatcggtggtgttgaattcgccccgcgtgtcgggcctgtgaccttccgtgtcagtggggaacgccgttccattaccaacagcgtcctgtcctatggcgggatgaccgatgccctgaccggcaagaagtggggcggtgttgtcaccaaccacttccatggacaggtcgaggctacgctgggcaataccattgtgtatggtggtggtggttatgccatccagaccggccatcatgtccagagcaataccgaagtggaaggcgggctgggggcgaataccctggtttatcgcaaccgcaagcacgaagtgcgtgtcggcgtgaacctgacctatctcggctataagcataacgaggacttctatacttacgggcagggtggctacttctcgccgcagtcctattttgcggcaacggtgcccgtccggtattccgggcatagtggcctgtttgactgggatgtcaccgggtccatcgggtatcagttgtttcatgaacacagttcggccttcttcccgaccaatcctgtgtatcaggcccttgcgaacgggctggcgggtgtatctactgctgaactatccttggaatcggccaggtatcccggcgatgatgtcggtagccttgttggtggtttcgatggcagggtgggttatcgcgtcagccattcgctgcgccttgatctgtccggtcgtttccagaaggctggaaactgggatgagggcggcgccatgatctcggctcactatcttattatggaccagtaatgacaatttttgagaaaaaaccggatttcaccctgtttcttcagaccctgtcatgggaaattgatgatcaggtcgggatcgaggtcaggaacgagctcctgcgtgaggtcggacggggcatgggcacgcgcatcatgccgccgccgtgccagaccgtggacaagctgcagatcgaactgaacgcgcttctggccctgatcggctggggcaccgtgacgctcgaactcctcagcgaggaccagtccctgcgcatcgtgcatgaaaacctgccgcaggttggcagcgcgggcgaaccttcgggcacgtggctggctccggtgctggaggggctgtatggccgctgggtgacgtcgcaggcgggtgcgtttggtgattatgtcgttacacgcgatgtggacgccgaggatctgaatgctgttccgcgtcagaccatcatcatgtacatgcgcgtgcgcagttccgcgacctga BBa_K571002_sequence 1 atgtcggtcatggcggcgatgggaggggcgcaggtgctttcatccaccggtgcgttcgcagacaccgcccccgatgcggtcgcgcagcaatgggccatcttccgcgccaagtatcttcgtcccagcggacgtgtcgtggatacgggcaatggtggcgaatcccatagtgaggggcagggctatggcatgctctttgccgcgtcggcgggggaccttgcgtcgttccagtcgatgtggatgtgggcgcgcaccaacctgcagcataccaatgacaagctgttttcctggcggttcctcaaggggcatcagcccccggtgcccgacaagaacaatgccacagatggcgacctgctgatcgcgcttgcgcttggtcgtgcgggcaagcgtttccagcgccccgattacattcaggacgccatggccatttatggcgatgtgctgaacctgatgacgatgaaggcgggaccgtatgtcgtcctcatgcccggtgctgtcggctttaccaagaaggacagcgtgatcctcaacctgtcctattacgtcatgccctcgctgctgcaggcgttcgaccttacggccgacccgcgctggcgtcaggtgatggaagacgggattcgccttgtttccgccggccgtttcgggcagtggcgcctgccccccgactggctggcggtgaatcgcgccaccggtgcgctgtcgatcgcatcgggatggccgccgcgcttttcctatgatgcgattcgggtgccgctttatttttattgggcgcatatgctggcgccgaacgtgttggctgatttcacccgattctggaataatttcggggctaatgccctgccaggatgggttgatctgacaacaggggcgcgttcgccgtacaacgccccgcctggatatcttgctgttgccgaatgcacggggcttgattctgccggggaactcccgacactggatcatgcgcccgattattattccgcagcgttgacgctgctcgtttacatcgcgcgggcggaggagactataaagtgagtgcttcagggtctgatgaggtggctgggggagggcaggctggaagtccgcaggattttcagcgggtcctgcgttcttttggtgtcgaaggtgggcagtattcctaccggccgtttgttgaccgttcctttgatgtgacaggcgtgcccgaggctgttgaaaggcacttcgatcaggcggagcatgacacggcggttgaggagcaggtcactcccgcgccacaaatcgcggtcgcaccgccaccgccgccagtcgttcctgacccgcccgccatcgtgacggaaaccgcgcccccgccgcctgtcgtggtcagcgctccggtcacgtatgaacccccggctgccgccgtgccggcagagcctcccgttcaggaagcccccgtgcaggcggcgccggttccccccgcgcctgtgcccccgattgcggagcaggctcctcccgcggcgccggacccggcatccgtgccgtatgcgaacgtcgcggcagcacccgttccacctgatcccgcaccggttacgcctgcgccgcaggcgcgcgtgacggggccgaacacccgtatggtggagcccttttcccgcccgcaggtccgcacggtgcaggagggggcaaccccgtcacgtgtaccttcgcgttcaatgaacgctttcccccgcacatcagcatcgtccataagtgagcgtccggtggacaggggtgttgccgatgaatggagtcctgttccgaaggcacgcctcagcccgcgggagcgtccgcgtcccggcgatctgagctttttctttcaggggatgcgcgacacccgtgatgaaaagaagttctttcccgtggcgtccacgcgatcagttcgttctaatgtttccaggatgaccagcatgaccaagacagacacgaattcctctcaggcttctcgtcccggcagccccgtcgcctcgcctgatgggtcgcccacaatggccgaagtgttcatgacgctgggtggtcgtgcgacggaactcctcagcccccgtccttcgctgcgggaggcgctgttgcgtcgtcgtgaaaacgaagaagaatcctaaggccc BBa_K571007_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagttaaggaggtaaaaaaatactagatgccagaggttcggtcgtcaacgcagtcagagtcaggaatgtcacagtggatggggaaaattctctccattcgcggtgctgggctgattattggtgtttttggcctgtgtgcgctgattgcggctacgtccgtgaccctgccgccagaacagcagttgattgtggcatttgtatgtgtcgtgatcttttttattgtcggtcataagcccagccgtcggtcccagattttccttgaagtgctgtcagggctggtttcgctgcgctatctgacatggcgcctgacggaaacgctttcattcgatacatggttgcagggtctgcttgggacaatgcttctggtggcggaactttacgccctgatgatgctgttcctcagctacttccagacgatcgcgccgctgcatcgtgcgcctctgccgctgccgccgaaccctgacgaatggcccacggtcgatatcttcgtcccgacctataacgaagaactgagcattgtccgcctgacggtgctgggatcactggggattgactggccaccggaaaaggtgcgggttcatatccttgatgacggccgtcgtcctgaattcgccgcctttgccgctgaatgtggcgcgaattatatcgcccgcccgacgaacgaacatgcaaaggccggtaatcttaactatgccattggtcataccgatggtgattacatcctgatctttgactgcgaccacgtcccgacccgcgccttcctgcagttgacaatgggctggatggtcgaagacccgaagatcgcgctgatgcagaccccgcatcacttctattcccccgacccgttccagcggaacctgtcggctggttatcgtaccccgcccgagggcaacctgttttatggcgtggtgcaggatggcaacgatttctgggatgcgaccttcttttgcgggtcatgtgcaatcctgcgtcgcacggcgattgagcagatcggcggctttgcgacccagaccgtgaccgaagacgcgcataccgcactcaagatgcagcgtctgggctggtccacggcctatctgcgtatcccgcttgccggtggtctcgcgacggaacgcctgatcctgcatatcggacagcgcgtgcgctgggcgcgtgggatgctgcagatcttccgcatcgacaatcctctgttcgggcgtggcctgtcatgggggcagcggctttgttacctgtcggccatgacgtcgttcctgttcgctgtcccgcgcgtcatcttcctgagctccccgctggcgttcctgttctttgggcagaacatcattgccgcgtcgccgctcgcgctgctggcctatgccatcccgcatatgttccacgccgtcggcacggcgtcgaagatcaacaagggctggcgctactccttctggagtgaggtctatgaaaccaccatggcgctgttcctggtgcgcgtgacgattgtcaccctgctcagcccttcacgtgggaagttcaacgtgacggacaagggcgggttgcttgaaaaaggttatttcgaccttggcgccgtctatccgaacatcatccttggcctgatcatgttcggcggtctggcgcgtggtgtctatgaactgtctttcggccatctcgaccagatcgccgaacgtgcctacctgctgaactccgcttgggcgatgctcagcctcatcatcatccttgcggccatcgccgtggggcgtgaaacacagcagaaacgcaacagtcatcgcatccccgcaaccatcccggtggaagtggcgaatgccgatgggtccatcatcgtgacgggcgtgaccgaggacctgtccatgggtggggccgcggtaaagatgtcatggcctgcgaagctgtcggggccgacgccggtttatatccgtactgtccttgatggagaggaactgatcctgcccgccaggatcatccgtgctggcaacgggcgggggatcttcatctggacgattgataacctgcagcaggaattctcggttatccgcctggtgttcggccgtgccgacgcatgggttgactggggcaattacaaggccgaccgcccgctgctcagcctcatggacatggttctcagcgtcaagggcctgttccgttcaagcggcgacatcgtccatcgcagttccccaaccaagccttcggctggcaatgccctgtctgacgatacgaacaacccgtcacgcaaggagcgtgtgctgaagggaaccgtgaaaatggtttcgcttctggcgctgctgacatttgcttcctcggcacaggcggcgtcagcgcccagggccgtcgcggcgaaggccccggcccatcagcccgaagcctctgacctgccgccgttgcccgcgctcctgccggccaccagcggtgcggcgcaggcgggttcgggcgatgccggcgccgatggacccggcagccccacgggccagcccctggcggccgacagtgccgatgcgctggtggaaaatgcggaaaatacgtccgatacggcgactgtccataattataccctcaaggatcttggcgccgcagggtccatcacaatgcgtggccttgccccgttgcaggggatcgagttcgggattccctctgaccagcttgtgacgtccgcgcgccttgtgctgtcgggttcgatgtcgcccaacctgcgtccggaaaccaattcggtcacaatgacgctgaacgagcagtatatcggcacgttgcgtcccgacccggcgcacccgacattcggccccatgtcgttcgagatcaacccgatcttcttcgtcagcggaaaccgtctgaacttcaacttcgcctccgggtcgaagggatgttcggacatcacgaacgatacgctgtgggccacgatctcgcagaactcgcagcttcagatcacgacaatcgcgctgccgccgcgtcgcctgctgtcacgtctgccgcagcccttctatgacaagaacgtgcgccagcatgttacggtcccgatggtgctggcgcagacctatgacccgcagatactcaagtccgcggggattctcgcttcgtggtttggcaagcagacggacttcctcggcgtgacgttcccggtgtcgtccaccatcccacagagtggcaacgccatcctgatcggcgtggccgatgaactgccgaccagcttcgggcggccgcaggtcaatggcccggcggttctggaactgccgaacccgtcggatgcaaatgccacgatcctggtggtgacggggcgtgaccgtgatgaggttattaccgcgagcaagggcatcgccttcgcgtctgctcccctgccgaccgacagccatatggatgtcgcgccggtcgatatcgccccgcgcaagcccaatgacgcgccatcttttatcgcgatggaccatccggtgcgctttggcgaccttgtaacggcaagcaagctacagggaaccggctttacgtccggtgtgctgtcggttccgttccgcattccgcccgacctttatacgtggcgtaaccgcccgtacaagatgcaggtacgtttccgttcccccgcgggggaggcgaaggatgtcgagaagtcacgtctcgatgtcgggatcaacgaggtttacctgcattcctatccgctgcgcgaaacgcatggcctgattggcgcggttttgcagggtgtcggccttgcccgccctgcaagcggcatgcaggtgcatgatctcgacgtaccgccgtggaccgtgttcgggcaggatcagttgaacttctactttgacgcgatgccccttgcgcgcggaatctgtcaaagtggcgcggcaaacaatgcattccatcttgggcttgacccggattccaccattgatttttcccgtgcccatcacatcgcccagatgcccaaccttgcctatatggcgacggtcggttttcctttcaccacctatgccgatctgtcgcagacggcggttgttctgcctgaacacccgaatgccgcgactgttggcgcctatcttgacctgatggggttcatgggcgcggcgacgtggtatccggttgcgggcgtggacattgtgtcggccgatcatgtcagtgacgttgcggaccgtaacctgctggtgatttcgacgctggcgacaagtggcgagatcgcgccgctgctgtcacgttcatcctacgaagtggcggatggtcatttgcgcacggtgtcgcatgcgtccgcgctggataacgcgatcaaggcggtcgacgatccgctgacggctttccgcgaccgtgacagcaagccgcaggatgtcgatacccccctgacgggtggtgtcggcgcgatgatcgaggcggaatcccccctgacggcgggccgcaccgttctggcgctgctgtcgtctgacggtgcggggctgaacaacctccttcagatgttgggggagcgcaagaagcaggcgaatatccagggggacctggttgttgcccatggcgaggacctgtcttcgtaccgcacttcgccggtctatacgatcggcaccctgccgctctggctgtggccggactggtatatgcacaacagaccggttcgtgtgctgcttgtcggcctgttgggatgtattctgatcgtcagtgttcttgcacgggctctggcgcggcatgcggcccggcgtttcaagcagcttgaggatgagaggcgcaagtcgtgatactagatgcttcgtccctgtccgccggtcttctcgcaacgacctgcgtcgcaggtctgttgctgcaggcgaacggcgcacgggcacagcaggcggcagaggcgcaggccccggccagcagcaccaccatgatgcaggcagctaccgttgcccccgcccagagtgggcaggccgcggtggtgcagcggctggtgcagcaggcccgtttctggatgcagcagcaccagtatgaaaatgcgcgccagtccctgcagagtgctgcgcgacttgcaccggattccgttgatctgctggaggccgagggcgaatatcagtcgcatatcggcaatcgtgatgccgcccttgatacgcagcgtcgcctgcatcaggccgcaccaggcagcacgtatgaaagccagttgaacgacctgctgcatgaacaggcgatttcccagccggaccttgcgcatgcgcgctcgcttgccgcatccgggcacagcgatcaggcggtggaagcgtaccagcacctgttcaacggttcgacgcccacgccctcgctcgcggttgaatattaccagacgctggctgacgtctcggggcaggccggcacggcacaggatgggctgatccgtctggtcaaggccaatccttccgatttccgggcgcaactggcccttgcgcaggtcctgacctatcagcccggcacccggatggaggggctgcagcggcttcaggcgctccagaagtaccagtcttccgccccggtggaggctgcgacggcggaaaaatcatatcgccagacgctgtcatggttaccggttacacccgaaacactgccgttgatgcagaaatggctggacgcacatccatccgacagcgcattgcggacccatatggcagaaccggcaggcgggccgccggataaaggcgcgttggcgcggcaggacgggttcaaggcgttgaacgccggacgtctgtccgcagcccaggccgcgttccagagcgcactgaacctgaatgccaaggacggcgatgccctgggcggccttggccttgtcgccatgcgcgcgggccataacgaggaagcgcatcgttacctcgaagacgcgatcgcggctgatcccaagaatgcggcgcactggcgtccggcactggctggcatggccgtgggcgaggaatatggcagcgtccgtcgcctgatcgccagtggacagacacaggaagccgaacagcgcctgatgacgctggcgcgtcagcccggacagtccgagggcgcgacccttatgctggcggatttgcagcgcagcacgggccagaccggcgaggccgagcgcaattaccgggcgatcctggcgcgcaatggtgacaatcccatcgcactgatgggactggcccgtgtgctgatgggggaaggccaggagaacgaagcgaacgccctgctgtcgcgccttggtggtcgctatagcgatcaggtgcagcagatcgaggtttcgggtatcatggccgaagctgcccgcacgtcggattcagcgcagaaggtcagccttctgcggcaggccatgaccaaggccccggatgacccgtggttgcgcatcaaccttgccaatgcgctgcaacagcaaggtgacagcgcggaagcggccaatgtcatgcgtccgcttctgaccagcccgcgtacgccggccgattatcaggcggcgatcctgtatgcgtccggcaatggcaatgatacgctggcacgccgtttgcttgcgggactgtcgccggatgactattccccggccatccgcaccattgccgatgaaatggcgatcaaggccgatctggccagccgcctttcgatggtttcgaatcccacgccgcttgtgcgcgaggctctggcggcgccggacccgacgggcgcgcgtggtgtggcggtggccgacctgttccggcagcgtggtgacatgctgcatgcgcatatggcgctgcgcatcgcctccacgcgcaatattgacctgacgaccgaacagcggctggcctatgccaccgaatatatgaagatcagcaatcccgttgccgcagcgcgcctgcttgcaccgttgggcgatggtagtggaaccgcaaccggttccgcgatgtcgccggatcagcggcagacgctgatgcagttgcgcatgggcatttcggtggcacagtcggacctgctgaaccagcgtggcgatcaggcggcggcgtgtgaccaccttgcgcccgcgttgcaggccgatccagaggcgacatcccccaagcttgcgttggcgcgactgtataacgggcgtggcaagtatgggcacgcgctggacatcgaccttgctgttcttcggcataatccgcaggaccttgatgcgcggcaggccgccgtgcaggccgcggcgaatgacggcaaggacaacctggccatgcaactggcgcaagatggggtccagcagtcgccgatggatgcgcgcagctggcttggcatggcggtggcggaccacgctgtgggccatggcgaccggacgcttgctgacctgcgccgggcgtatgaattgcgcctgcagcagctcaagatcagtcggggtgatgccatcggcggtgatgaaacgcaggcgaccgcgccgccgacggccaatccgttccgtcgcgatgcatacgggcatgcgctgtcgctgggtgcgcccccgggtgagaacgggtacagcacggcaggaagcgtgcccgagatttcggaccagatgctgtcctccatcaacgggcagatccataccctgtccgaagacatggcgccgtctgttgatgccggccttggcttccgtgtccgttccggcaccccgggcatgggcgcgttgaccgaggcatccgtgccgatcgtcgggcgcatcccgctgcaggctggtacttcggcgctgacattcacggccacgccaaccttcctgacgtcggggcacctgccgcagaccgggtatgatataccgcgtttcggcaccaacctgttcgcgctggaacggaacctgcagaaccagaacaacagcgcggaacatcgcatcaataccgatacgatcgggcgcgaggcaggtgtggcgcctgatgtccgctttgccaataactgggtcagtgccgatgtcggtgcctcgcctctgggctttacgctgccgaacgtgatcggtggtgttgaattcgccccgcgtgtcgggcctgtgaccttccgtgtcagtggggaacgccgttccattaccaacagcgtcctgtcctatggcgggatgaccgatgccctgaccggcaagaagtggggcggtgttgtcaccaaccacttccatggacaggtcgaggctacgctgggcaataccattgtgtatggtggtggtggttatgccatccagaccggccatcatgtccagagcaataccgaagtggaaggcgggctgggggcgaataccctggtttatcgcaaccgcaagcacgaagtgcgtgtcggcgtgaacctgacctatctcggctataagcataacgaggacttctatacttacgggcagggtggctacttctcgccgcagtcctattttgcggcaacggtgcccgtccggtattccgggcatagtggcctgtttgactgggatgtcaccgggtccatcgggtatcagttgtttcatgaacacagttcggccttcttcccgaccaatcctgtgtatcaggcccttgcgaacgggctggcgggtgtatctactgctgaactatccttggaatcggccaggtatcccggcgatgatgtcggtagccttgttggtggtttcgatggcagggtgggttatcgcgtcagccattcgctgcgccttgatctgtccggtcgtttccagaaggctggaaactgggatgagggcggcgccatgatctcggctcactatcttattatggaccagtaatgacaatttttgagaaaaaaccggatttcaccctgtttcttcagaccctgtcatgggaaattgatgatcaggtcgggatcgaggtcaggaacgagctcctgcgtgaggtcggacggggcatgggcacgcgcatcatgccgccgccgtgccagaccgtggacaagctgcagatcgaactgaacgcgcttctggccctgatcggctggggcaccgtgacgctcgaactcctcagcgaggaccagtccctgcgcatcgtgcatgaaaacctgccgcaggttggcagcgcgggcgaaccttcgggcacgtggctggctccggtgctggaggggctgtatggccgctgggtgacgtcgcaggcgggtgcgtttggtgattatgtcgttacacgcgatgtggacgccgaggatctgaatgctgttccgcgtcagaccatcatcatgtacatgcgcgtgcgcagttccgcgacctgatactagatgtcggtcatggcggcgatgggaggggcgcaggtgctttcatccaccggtgcgttcgcagacaccgcccccgatgcggtcgcgcagcaatgggccatcttccgcgccaagtatcttcgtcccagcggacgtgtcgtggatacgggcaatggtggcgaatcccatagtgaggggcagggctatggcatgctctttgccgcgtcggcgggggaccttgcgtcgttccagtcgatgtggatgtgggcgcgcaccaacctgcagcataccaatgacaagctgttttcctggcggttcctcaaggggcatcagcccccggtgcccgacaagaacaatgccacagatggcgacctgctgatcgcgcttgcgcttggtcgtgcgggcaagcgtttccagcgccccgattacattcaggacgccatggccatttatggcgatgtgctgaacctgatgacgatgaaggcgggaccgtatgtcgtcctcatgcccggtgctgtcggctttaccaagaaggacagcgtgatcctcaacctgtcctattacgtcatgccctcgctgctgcaggcgttcgaccttacggccgacccgcgctggcgtcaggtgatggaagacgggattcgccttgtttccgccggccgtttcgggcagtggcgcctgccccccgactggctggcggtgaatcgcgccaccggtgcgctgtcgatcgcatcgggatggccgccgcgcttttcctatgatgcgattcgggtgccgctttatttttattgggcgcatatgctggcgccgaacgtgttggctgatttcacccgattctggaataatttcggggctaatgccctgccaggatgggttgatctgacaacaggggcgcgttcgccgtacaacgccccgcctggatatcttgctgttgccgaatgcacggggcttgattctgccggggaactcccgacactggatcatgcgcccgattattattccgcagcgttgacgctgctcgtttacatcgcgcgggcggaggagactataaagtgagtgcttcagggtctgatgaggtggctgggggagggcaggctggaagtccgcaggattttcagcgggtcctgcgttcttttggtgtcgaaggtgggcagtattcctaccggccgtttgttgaccgttcctttgatgtgacaggcgtgcccgaggctgttgaaaggcacttcgatcaggcggagcatgacacggcggttgaggagcaggtcactcccgcgccacaaatcgcggtcgcaccgccaccgccgccagtcgttcctgacccgcccgccatcgtgacggaaaccgcgcccccgccgcctgtcgtggtcagcgctccggtcacgtatgaacccccggctgccgccgtgccggcagagcctcccgttcaggaagcccccgtgcaggcggcgccggttccccccgcgcctgtgcccccgattgcggagcaggctcctcccgcggcgccggacccggcatccgtgccgtatgcgaacgtcgcggcagcacccgttccacctgatcccgcaccggttacgcctgcgccgcaggcgcgcgtgacggggccgaacacccgtatggtggagcccttttcccgcccgcaggtccgcacggtgcaggagggggcaaccccgtcacgtgtaccttcgcgttcaatgaacgctttcccccgcacatcagcatcgtccataagtgagcgtccggtggacaggggtgttgccgatgaatggagtcctgttccgaaggcacgcctcagcccgcgggagcgtccgcgtcccggcgatctgagctttttctttcaggggatgcgcgacacccgtgatgaaaagaagttctttcccgtggcgtccacgcgatcagttcgttctaatgtttccaggatgaccagcatgaccaagacagacacgaattcctctcaggcttctcgtcccggcagccccgtcgcctcgcctgatgggtcgcccacaatggccgaagtgttcatgacgctgggtggtcgtgcgacggaactcctcagcccccgtccttcgctgcgggaggcgctgttgcgtcgtcgtgaaaacgaagaagaatcctaaggccc BBa_K571004_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagttaaggaggtaaaaaaa BBa_K571000_sequence 1 atgccagaggttcggtcgtcaacgcagtcagagtcaggaatgtcacagtggatggggaaaattctctccattcgcggtgctgggctgattattggtgtttttggcctgtgtgcgctgattgcggctacgtccgtgaccctgccgccagaacagcagttgattgtggcatttgtatgtgtcgtgatcttttttattgtcggtcataagcccagccgtcggtcccagattttccttgaagtgctgtcagggctggtttcgctgcgctatctgacatggcgcctgacggaaacgctttcattcgatacatggttgcagggtctgcttgggacaatgcttctggtggcggaactttacgccctgatgatgctgttcctcagctacttccagacgatcgcgccgctgcatcgtgcgcctctgccgctgccgccgaaccctgacgaatggcccacggtcgatatcttcgtcccgacctataacgaagaactgagcattgtccgcctgacggtgctgggatcactggggattgactggccaccggaaaaggtgcgggttcatatccttgatgacggccgtcgtcctgaattcgccgcctttgccgctgaatgtggcgcgaattatatcgcccgcccgacgaacgaacatgcaaaggccggtaatcttaactatgccattggtcataccgatggtgattacatcctgatctttgactgcgaccacgtcccgacccgcgccttcctgcagttgacaatgggctggatggtcgaagacccgaagatcgcgctgatgcagaccccgcatcacttctattcccccgacccgttccagcggaacctgtcggctggttatcgtaccccgcccgagggcaacctgttttatggcgtggtgcaggatggcaacgatttctgggatgcgaccttcttttgcgggtcatgtgcaatcctgcgtcgcacggcgattgagcagatcggcggctttgcgacccagaccgtgaccgaagacgcgcataccgcactcaagatgcagcgtctgggctggtccacggcctatctgcgtatcccgcttgccggtggtctcgcgacggaacgcctgatcctgcatatcggacagcgcgtgcgctgggcgcgtgggatgctgcagatcttccgcatcgacaatcctctgttcgggcgtggcctgtcatgggggcagcggctttgttacctgtcggccatgacgtcgttcctgttcgctgtcccgcgcgtcatcttcctgagctccccgctggcgttcctgttctttgggcagaacatcattgccgcgtcgccgctcgcgctgctggcctatgccatcccgcatatgttccacgccgtcggcacggcgtcgaagatcaacaagggctggcgctactccttctggagtgaggtctatgaaaccaccatggcgctgttcctggtgcgcgtgacgattgtcaccctgctcagcccttcacgtgggaagttcaacgtgacggacaagggcgggttgcttgaaaaaggttatttcgaccttggcgccgtctatccgaacatcatccttggcctgatcatgttcggcggtctggcgcgtggtgtctatgaactgtctttcggccatctcgaccagatcgccgaacgtgcctacctgctgaactccgcttgggcgatgctcagcctcatcatcatccttgcggccatcgccgtggggcgtgaaacacagcagaaacgcaacagtcatcgcatccccgcaaccatcccggtggaagtggcgaatgccgatgggtccatcatcgtgacgggcgtgaccgaggacctgtccatgggtggggccgcggtaaagatgtcatggcctgcgaagctgtcggggccgacgccggtttatatccgtactgtccttgatggagaggaactgatcctgcccgccaggatcatccgtgctggcaacgggcgggggatcttcatctggacgattgataacctgcagcaggaattctcggttatccgcctggtgttcggccgtgccgacgcatgggttgactggggcaattacaaggccgaccgcccgctgctcagcctcatggacatggttctcagcgtcaagggcctgttccgttcaagcggcgacatcgtccatcgcagttccccaaccaagccttcggctggcaatgccctgtctgacgatacgaacaacccgtcacgcaaggagcgtgtgctgaagggaaccgtgaaaatggtttcgcttctggcgctgctgacatttgcttcctcggcacaggcggcgtcagcgcccagggccgtcgcggcgaaggccccggcccatcagcccgaagcctctgacctgccgccgttgcccgcgctcctgccggccaccagcggtgcggcgcaggcgggttcgggcgatgccggcgccgatggacccggcagccccacgggccagcccctggcggccgacagtgccgatgcgctggtggaaaatgcggaaaatacgtccgatacggcgactgtccataattataccctcaaggatcttggcgccgcagggtccatcacaatgcgtggccttgccccgttgcaggggatcgagttcgggattccctctgaccagcttgtgacgtccgcgcgccttgtgctgtcgggttcgatgtcgcccaacctgcgtccggaaaccaattcggtcacaatgacgctgaacgagcagtatatcggcacgttgcgtcccgacccggcgcacccgacattcggccccatgtcgttcgagatcaacccgatcttcttcgtcagcggaaaccgtctgaacttcaacttcgcctccgggtcgaagggatgttcggacatcacgaacgatacgctgtgggccacgatctcgcagaactcgcagcttcagatcacgacaatcgcgctgccgccgcgtcgcctgctgtcacgtctgccgcagcccttctatgacaagaacgtgcgccagcatgttacggtcccgatggtgctggcgcagacctatgacccgcagatactcaagtccgcggggattctcgcttcgtggtttggcaagcagacggacttcctcggcgtgacgttcccggtgtcgtccaccatcccacagagtggcaacgccatcctgatcggcgtggccgatgaactgccgaccagcttcgggcggccgcaggtcaatggcccggcggttctggaactgccgaacccgtcggatgcaaatgccacgatcctggtggtgacggggcgtgaccgtgatgaggttattaccgcgagcaagggcatcgccttcgcgtctgctcccctgccgaccgacagccatatggatgtcgcgccggtcgatatcgccccgcgcaagcccaatgacgcgccatcttttatcgcgatggaccatccggtgcgctttggcgaccttgtaacggcaagcaagctacagggaaccggctttacgtccggtgtgctgtcggttccgttccgcattccgcccgacctttatacgtggcgtaaccgcccgtacaagatgcaggtacgtttccgttcccccgcgggggaggcgaaggatgtcgagaagtcacgtctcgatgtcgggatcaacgaggtttacctgcattcctatccgctgcgcgaaacgcatggcctgattggcgcggttttgcagggtgtcggccttgcccgccctgcaagcggcatgcaggtgcatgatctcgacgtaccgccgtggaccgtgttcgggcaggatcagttgaacttctactttgacgcgatgccccttgcgcgcggaatctgtcaaagtggcgcggcaaacaatgcattccatcttgggcttgacccggattccaccattgatttttcccgtgcccatcacatcgcccagatgcccaaccttgcctatatggcgacggtcggttttcctttcaccacctatgccgatctgtcgcagacggcggttgttctgcctgaacacccgaatgccgcgactgttggcgcctatcttgacctgatggggttcatgggcgcggcgacgtggtatccggttgcgggcgtggacattgtgtcggccgatcatgtcagtgacgttgcggaccgtaacctgctggtgatttcgacgctggcgacaagtggcgagatcgcgccgctgctgtcacgttcatcctacgaagtggcggatggtcatttgcgcacggtgtcgcatgcgtccgcgctggataacgcgatcaaggcggtcgacgatccgctgacggctttccgcgaccgtgacagcaagccgcaggatgtcgatacccccctgacgggtggtgtcggcgcgatgatcgaggcggaatcccccctgacggcgggccgcaccgttctggcgctgctgtcgtctgacggtgcggggctgaacaacctccttcagatgttgggggagcgcaagaagcaggcgaatatccagggggacctggttgttgcccatggcgaggacctgtcttcgtaccgcacttcgccggtctatacgatcggcaccctgccgctctggctgtggccggactggtatatgcacaacagaccggttcgtgtgctgcttgtcggcctgttgggatgtattctgatcgtcagtgttcttgcacgggctctggcgcggcatgcggcccggcgtttcaagcagcttgaggatgagaggcgcaagtcgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z