BBa_K572005 1 BBa_K572005 Proteorhodopsin 2011-09-17T11:00:00Z 2015-05-08T01:12:44Z Source Organism : Marine gamma proteobacterium EBAC31A08 We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with proteorhodopsin gene. Proteorhodopsin, a photon dependent proton pump, is a photoactive retinylidene protein. A transmembrane protein homologous to bacteriorhodopsin in Archaea creates a proton gradient across the cell membrane in many marine organisms. It has been demonstrated that photophosphorylation occurs with the help of transmembrane ATP Synthase on expression of Proteorhodopsin gene in Escherichia coli* *Martinez A, Bradley AS, Waldbauer JR, Summons RE, DeLong EF (2007). "Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host". PNAS 104 (13): 5590???5595. Bibcode 2007PNAS..104.5590M. doi:10.1073/pnas.0611470104. PMC 1838496. PMID 17372221. false false _743_ 0 6058 9 It's complicated false The gene had E,S and P sites. Thus, we used site directed mutagenesis to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone. false Avaneesh T annotation2155683 1 Stop range2155683 1 755 758 annotation2155682 1 Start range2155682 1 1 3 BBa_K572005_sequence 1 atgaaattattactgatattaggtagtgttattgcacttcctacatttgccgcaggtggtggtgaccttgatgctagtgattacactggtgtttctttttggttagttactgctgctttattagcatctactgtatttttctttgttgaaagagatagagtttctgcaaaatggaaaacatcattaactgtatctggtcttgttactggtattgctttctggcattacatgtacatgagaggggtatggattgaaactggtgattcgccaactgtatttagatacattgattggttactaacagttcctctattaatatgtgagttctacttaattcttgctgctgcaactaatgttgctggatcattatttaagaaactgctagttggttctcttgttatgcttgtgtttggttacatgggtgaagcaggaatcatggctgcatggcctgcattcattattgggtgtttagcttgggtatacatgatttatgaattatgggctggagaaggaaaatctgcatgtaatactgcaagtcctgctgtgcaatcagcttacaacacaatgatgtatattatcatctttggttgggcgatttatcctgtaggttatttcacaggttacctgatgggtgacggtggatcagctcttaacttaaaccttatctataaccttgctgactttgttaacaagattctatttggtttaattatatggaatgttgctgttaaagaatcttctaatgcttaactaataac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z