BBa_K572005 1 BBa_K572005 Proteorhodopsin 2011-09-17T11:00:00Z 2015-05-08T01:12:44Z Source Organism : Marine gamma proteobacterium EBAC31A08 We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with proteorhodopsin gene. Proteorhodopsin, a photon dependent proton pump, is a photoactive retinylidene protein. A transmembrane protein homologous to bacteriorhodopsin in Archaea creates a proton gradient across the cell membrane in many marine organisms. It has been demonstrated that photophosphorylation occurs with the help of transmembrane ATP Synthase on expression of Proteorhodopsin gene in Escherichia coli* *Martinez A, Bradley AS, Waldbauer JR, Summons RE, DeLong EF (2007). "Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host". PNAS 104 (13): 5590???5595. Bibcode 2007PNAS..104.5590M. doi:10.1073/pnas.0611470104. PMC 1838496. PMID 17372221. false false _743_ 0 6058 9 It's complicated false The gene had E,S and P sites. Thus, we used site directed mutagenesis to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone. false Avaneesh T annotation2155683 1 Stop range2155683 1 755 758 annotation2155682 1 Start range2155682 1 1 3 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_I712074 1 BBa_I712074 T7 promoter (strong promoter from T7 bacteriophage) 2007-10-21T11:00:00Z 2015-08-31T04:07:46Z T7 bacteriophage T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes. false false _130_ 0 1856 9 In stock false true Rok Gaber BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K572008 1 BBa_K572008 Proteorhodopsin with T7 Constitutive Promoter 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z BBa_I712074 Bba_K572006 .. false false _743_ 0 8355 9 Not in stock false .. false Chaitra Prabhakara component2150812 1 BBa_I712074 component2150823 1 BBa_K572006 annotation2150823 1 BBa_K572006 range2150823 1 55 967 annotation2150812 1 BBa_I712074 range2150812 1 1 46 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K572006 1 BBa_K572006 Proteorhodopsin Generator 2011-09-17T11:00:00Z 2015-05-08T01:12:44Z BBa_K572005 - Proteorhodopsin (GPR) BBa_B0034 - RBS BBa_B0015 - Double Terminator To study the expression and function of proteorhodopsin under various promoters false false _743_ 0 8355 9 Not in stock false -- NONE -- false Chaitra Prabhakara component2132732 1 BBa_B0015 component2132724 1 BBa_B0034 component2132725 1 BBa_K572005 annotation2132732 1 BBa_B0015 range2132732 1 785 913 annotation2132724 1 BBa_B0034 range2132724 1 1 12 annotation2132725 1 BBa_K572005 range2132725 1 19 776 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K572006_sequence 1 aaagaggagaaatactagatgaaattattactgatattaggtagtgttattgcacttcctacatttgccgcaggtggtggtgaccttgatgctagtgattacactggtgtttctttttggttagttactgctgctttattagcatctactgtatttttctttgttgaaagagatagagtttctgcaaaatggaaaacatcattaactgtatctggtcttgttactggtattgctttctggcattacatgtacatgagaggggtatggattgaaactggtgattcgccaactgtatttagatacattgattggttactaacagttcctctattaatatgtgagttctacttaattcttgctgctgcaactaatgttgctggatcattatttaagaaactgctagttggttctcttgttatgcttgtgtttggttacatgggtgaagcaggaatcatggctgcatggcctgcattcattattgggtgtttagcttgggtatacatgatttatgaattatgggctggagaaggaaaatctgcatgtaatactgcaagtcctgctgtgcaatcagcttacaacacaatgatgtatattatcatctttggttgggcgatttatcctgtaggttatttcacaggttacctgatgggtgacggtggatcagctcttaacttaaaccttatctataaccttgctgactttgttaacaagattctatttggtttaattatatggaatgttgctgttaaagaatcttctaatgcttaactaataactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K572005_sequence 1 atgaaattattactgatattaggtagtgttattgcacttcctacatttgccgcaggtggtggtgaccttgatgctagtgattacactggtgtttctttttggttagttactgctgctttattagcatctactgtatttttctttgttgaaagagatagagtttctgcaaaatggaaaacatcattaactgtatctggtcttgttactggtattgctttctggcattacatgtacatgagaggggtatggattgaaactggtgattcgccaactgtatttagatacattgattggttactaacagttcctctattaatatgtgagttctacttaattcttgctgctgcaactaatgttgctggatcattatttaagaaactgctagttggttctcttgttatgcttgtgtttggttacatgggtgaagcaggaatcatggctgcatggcctgcattcattattgggtgtttagcttgggtatacatgatttatgaattatgggctggagaaggaaaatctgcatgtaatactgcaagtcctgctgtgcaatcagcttacaacacaatgatgtatattatcatctttggttgggcgatttatcctgtaggttatttcacaggttacctgatgggtgacggtggatcagctcttaacttaaaccttatctataaccttgctgactttgttaacaagattctatttggtttaattatatggaatgttgctgttaaagaatcttctaatgcttaactaataac BBa_K572008_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatgaaattattactgatattaggtagtgttattgcacttcctacatttgccgcaggtggtggtgaccttgatgctagtgattacactggtgtttctttttggttagttactgctgctttattagcatctactgtatttttctttgttgaaagagatagagtttctgcaaaatggaaaacatcattaactgtatctggtcttgttactggtattgctttctggcattacatgtacatgagaggggtatggattgaaactggtgattcgccaactgtatttagatacattgattggttactaacagttcctctattaatatgtgagttctacttaattcttgctgctgcaactaatgttgctggatcattatttaagaaactgctagttggttctcttgttatgcttgtgtttggttacatgggtgaagcaggaatcatggctgcatggcctgcattcattattgggtgtttagcttgggtatacatgatttatgaattatgggctggagaaggaaaatctgcatgtaatactgcaagtcctgctgtgcaatcagcttacaacacaatgatgtatattatcatctttggttgggcgatttatcctgtaggttatttcacaggttacctgatgggtgacggtggatcagctcttaacttaaaccttatctataaccttgctgactttgttaacaagattctatttggtttaattatatggaatgttgctgttaaagaatcttctaatgcttaactaataactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I712074_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgca BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z