BBa_K572009 1 BBa_K572009 Mouse Beta Carotene Dioxygenase 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z Source Organism : Marine gamma proteobacterium EBAC31A08. We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with dioxygenase gene. .. false true _743_ 0 7684 9 It's complicated false Forward Primer : 5???TTTACTACGAATTCGCGGCCGCTTCTAGATGGAGATAATATTTG 3??? Reverse Primer : 5???AATCGAACctgcagcggccgctactagtaTTAAAGACTTGAG 3??? Polymerase Chain reactions didn't work for a long time at various temperature conditions. Finally, increasing Magnesium Sulphate in the reaction resulted in bringing about the amplification. The following is the optimized PCR Setup : Template (116 ng/ul) = 0.25 ul 10mM dNTP = 1.0 ul 10x Pfu Buffer (without Magnesium Sulphate) = 2.0 ul + Primer (10 uM) = 1.0 ul - Primer (10 uM) = 1.0 ul Magnesium Sulphate - final concentration of 2.5 - 3 mM Pfu Pol = 1.25 u Nuclease Free Water - make the volume to 20ul false Kousik Sundararajan annotation2151503 1 Bc_dioxygenase range2151503 1 1 1701 annotation2151502 1 Start Codon range2151502 1 1 3 annotation2151504 1 Stop Codon range2151504 1 1699 1701 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K572010 1 BBa_K572010 Mouse Beta Carotene Dioxygenase Generator 2011-10-02T11:00:00Z 2015-05-08T01:12:44Z BBa_B0034 BBa_K572009 Bba_B0015 .. false false _743_ 0 7684 9 Not in stock false .. false Kousik Sundararajan component2151514 1 BBa_B0034 component2151525 1 BBa_B0015 component2151518 1 BBa_K572009 annotation2151518 1 BBa_K572009 range2151518 1 19 1719 annotation2151525 1 BBa_B0015 range2151525 1 1728 1856 annotation2151514 1 BBa_B0034 range2151514 1 1 12 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K572009_sequence 1 atggagataatatttggccagaataagaaagaacagctggagccagttcaggccaaagtgacaggcagcattccagcatggctgcaggggaccctgctccgaaacgggcccgggatgcacacagtgggagagagcaagtacaaccattggtttgatggcctggcccttctccacagtttctccatcagagatggggaggtcttctacaggagcaaatacctgcagagtgacacctacatcgccaacattgaggccaacagaatcgtggtgtctgagttcggaaccatggcctacccggacccctgcaaaaacatcttttccaaagctttctcctacttgtctcacaccatccccgacttcacagacaactgtctgatcaacatcatgaaatgtggagaagacttctatgcaaccacggagaccaactacatcaggaaaatcgacccccagaccctagagactttggagaaggttgattaccggaagtatgtggcggtaaacctggctacctcgcaccctcattatgacgaggctgggaatgtccttaacatgggcacatccgtcgtggacaaagggaggacaaaatacgtgatatttaagatccctgccacagtgccagacagcaagaagaaagggaagagtcccgtgaagcacgcggaagttttctgctccatttcctcccgctcgctgctctctcccagctactaccacagctttggtgtcacggagaactatgtggtgtttctggagcagccttttaagttggatatcctcaagatggccaccgcatacatgaggggagtgagctgggcttcctgtatgtcattcgacagggaggacaagacatacattcatatcatcgaccagaggaccaggaagcctgtgcctaccaagttctacacagatcccatggtggtcttccatcatgtcaatgcctacgaggaggacggctgtgtgctgtttgatgtgatcgcctatgaggacagcagcctctatcagctcttctacctggccaacctgaacaaggacttcgaggagaagtccaggctgacctcagtgcctaccctcaggaggtttgctgtgcccctccatgtggacaaggatgcagaagtgggctcaaatttagtcaaggtgtcatctacaactgcaacagccctgaaggagaaagacggccatgtctattgccagcccgaggtcctctacgaaggcctagagctccctcggataaattatgcttacaacgggaagccatatcgctacatctttgcagctgaagtacagtggagtccagtcccaaccaagatactgaaatatgacattctcacaaagtcctccttaaagtggtctgaggagagctgctggccagcagagcctctgtttgttcccacgccaggtgcgagggatgaagatgatggagtcattttatcagccatcgtctctacggatccccaaaagctgccttttttactcattctggatgccaaaagttttgcggaactggctcgcgcctctgttgatgcggacatgcacctggaccttcatggtttatttatcccagatgcagactggaatgcagtgaagcagactccagctgaaacgcaagaggttgaaaactcagatcatcccacagatccgacagcaccagaactgagccacagtgaaaatgacttcacagcgggtcatggtggctcaagtctttaa BBa_K572010_sequence 1 aaagaggagaaatactagatggagataatatttggccagaataagaaagaacagctggagccagttcaggccaaagtgacaggcagcattccagcatggctgcaggggaccctgctccgaaacgggcccgggatgcacacagtgggagagagcaagtacaaccattggtttgatggcctggcccttctccacagtttctccatcagagatggggaggtcttctacaggagcaaatacctgcagagtgacacctacatcgccaacattgaggccaacagaatcgtggtgtctgagttcggaaccatggcctacccggacccctgcaaaaacatcttttccaaagctttctcctacttgtctcacaccatccccgacttcacagacaactgtctgatcaacatcatgaaatgtggagaagacttctatgcaaccacggagaccaactacatcaggaaaatcgacccccagaccctagagactttggagaaggttgattaccggaagtatgtggcggtaaacctggctacctcgcaccctcattatgacgaggctgggaatgtccttaacatgggcacatccgtcgtggacaaagggaggacaaaatacgtgatatttaagatccctgccacagtgccagacagcaagaagaaagggaagagtcccgtgaagcacgcggaagttttctgctccatttcctcccgctcgctgctctctcccagctactaccacagctttggtgtcacggagaactatgtggtgtttctggagcagccttttaagttggatatcctcaagatggccaccgcatacatgaggggagtgagctgggcttcctgtatgtcattcgacagggaggacaagacatacattcatatcatcgaccagaggaccaggaagcctgtgcctaccaagttctacacagatcccatggtggtcttccatcatgtcaatgcctacgaggaggacggctgtgtgctgtttgatgtgatcgcctatgaggacagcagcctctatcagctcttctacctggccaacctgaacaaggacttcgaggagaagtccaggctgacctcagtgcctaccctcaggaggtttgctgtgcccctccatgtggacaaggatgcagaagtgggctcaaatttagtcaaggtgtcatctacaactgcaacagccctgaaggagaaagacggccatgtctattgccagcccgaggtcctctacgaaggcctagagctccctcggataaattatgcttacaacgggaagccatatcgctacatctttgcagctgaagtacagtggagtccagtcccaaccaagatactgaaatatgacattctcacaaagtcctccttaaagtggtctgaggagagctgctggccagcagagcctctgtttgttcccacgccaggtgcgagggatgaagatgatggagtcattttatcagccatcgtctctacggatccccaaaagctgccttttttactcattctggatgccaaaagttttgcggaactggctcgcgcctctgttgatgcggacatgcacctggaccttcatggtttatttatcccagatgcagactggaatgcagtgaagcagactccagctgaaacgcaagaggttgaaaactcagatcatcccacagatccgacagcaccagaactgagccacagtgaaaatgacttcacagcgggtcatggtggctcaagtctttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z