BBa_K572100 1 BBa_K572100 Light based screening plasmid backbone - pSB1Pc 2011-10-04T11:00:00Z 2015-05-08T01:12:44Z Source : pSB1C3 is modified. Proteorhodopsin replaces the antibiotic gene. Screening techniques available presently are all negatively related. The disadvantages of antibiotics are: 1. Principle of Negative Selection 2. Transformants carry a small but unnecessary risk of horizontal gene transfer to environmental microbes 3. Metabolic burden on the cells that significantly reduces the production of the desired protein Therefore, a novel selectable marker is needed which confers metabolic ???advantage??? to the transformants. This plasmid carries the gene proteorhodopsin. false false _743_ 0 8355 9 Not in stock false The reading frame had to be maintained. false Chaitra Prabhakara BBa_K572100_sequence 1 tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaaatgaaattattactgatattaggtagtgttattgcacttcctacatttgccgcaggtggtggtgaccttgatgctagtgattacactggtgtttctttttggttagttactgctgctttattagcatctactgtatttttctttgttgaaagagatagagtttctgcaaaatggaaaacatcattaactgtatctggtcttgttactggtattgctttctggcattacatgtacatgagaggggtatggattgaaactggtgattcgccaactgtatttagatacattgattggttactaacagttcctctattaatatgtgagttctacttaattcttgctgctgcaactaatgttgctggatcattatttaagaaactgctagttggttctcttgttatgcttgtgtttggttacatgggtgaagcaggaatcatggctgcatggcctgcattcattattgggtgtttagcttgggtatacatgatttatgaattatgggctggagaaggaaaatctgcatgtaatactgcaagtcctgctgtgcaatcagcttacaacacaatgatgtatattatcatctttggttgggcgatttatcctgtaggttatttcacaggttacctgatgggtgacggtggatcagctcttaacttaaaccttatctataaccttgctgactttgttaacaagattctatttggtttaattatatggaatgttgctgttaaagaatcttctaatgcttaacttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z