##gff-version 3
##sequence-region BBa_K574000 1 903
BBa_K574000	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K574000	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K574000	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K574000	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K574000	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K574000	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K574000	.	engineered_region	64	903	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K574000	.	terminator	775	854	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K574000	.	stem_loop	786	829	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K574000	.	engineered_region	775	854	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K574000	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K574000	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K574000	.	terminator	863	903	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K574000	.	polyA_site	890	903	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K574000	.	stem_loop	870	889	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K574000	.	engineered_region	863	903	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K574000	.	stop_codon	896	896	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K574000	.	CDS	82	741	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K574000	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K574000	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K574000	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K574000
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata