LOCUS       BBa_K574001             1890 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR regulated by 3OC6HSL
ACCESSION   BBa_K574001
VERSION     BBa_K574001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     terminator      851..979
                     /label=BBa_B0015
     misc_feature    1..979
                     /label=BBa_K145270
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1072..1688
                     /label=tetR
     misc_feature    1689..1722
                     /label=SsrA
     misc_feature    1729..1753
                     /label=Help:Barcodes
     CDS             1069..1728
                     /label=BBa_C0040
     stem_loop       1773..1816
                     /label=stem_loop
     misc_feature    1762..1841
                     /label=BBa_B0010
     terminator      1762..1841
                     /label=BBa_B0010
     stem_loop       1857..1876
                     /label=T7 TE
     misc_feature    1883..1883
                     /label=stop
     polyA_site      1877..1890
                     /label=polya
     misc_feature    1850..1890
                     /label=BBa_B0012
     terminator      1850..1890
                     /label=BBa_B0012
     misc_feature    1051..1890
                     /label=BBa_P0440
     misc_feature    988..1890
                     /label=BBa_K574000
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat gtccagatta
     1081 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
     1141 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
     1201 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
     1261 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
     1321 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
     1381 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
     1441 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
     1501 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
     1561 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
     1621 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
     1681 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
     1741 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1861 caccttcggg tgggcctttc tgcgtttata
//