##gff-version 3
##sequence-region BBa_K574004 1 797
BBa_K574004	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K574004	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K574004	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K574004	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K574004	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K574004	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K574004	.	engineered_region	63	797	.	+	0	ID=BBa_K081016;Name=BBa_K081016
BBa_K574004	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081016
BBa_K574004	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K574004	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K574004	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K574004	.	terminator	759	797	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081016
BBa_K574004	.	polyA_site	759	767	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K574004	.	polyA_site	790	797	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K574004	.	engineered_region	759	797	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K574004	.	stem_loop	768	789	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K574004	.	CDS	84	750	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081016
BBa_K574004	.	tag	687	719	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K574004	.	CDS	84	686	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K574004	.	engineered_tag	726	750	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
>BBa_K574004
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcag
gttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgt
actacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgta
catgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgt
ttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgtt
actccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtct
ggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaa
acccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctc
tggttgcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggt
ttttttt