##gff-version 3
##sequence-region BBa_K574005 1 1712
BBa_K574005	.	engineered_region	139	826	.	+	0	ID=BBa_K081009;Name=BBa_K081009
BBa_K574005	.	ribosome_entry_site	139	153	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081009
BBa_K574005	.	engineered_region	139	153	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K574005	.	ribosome_entry_site	146	150	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K574005	.	ribosome_entry_site	139	153	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K574005	.	CDS	160	826	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081009
BBa_K574005	.	tag	763	795	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K574005	.	engineered_tag	802	826	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K574005	.	CDS	160	762	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K574005	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K574005	.	engineered_region	835	1712	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_K574005	.	CDS	853	1575	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_K574005	.	terminator	1672	1712	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_K574005	.	engineered_region	1672	1712	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K574005	.	stop_codon	1705	1705	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K574005	.	stem_loop	1679	1698	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K574005	.	polyA_site	1699	1712	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K574005	.	terminator	1584	1663	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_K574005	.	stem_loop	1595	1638	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K574005	.	engineered_region	1584	1663	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K574005	.	ribosome_entry_site	835	846	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_K574005	.	conserved_region	839	842	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K574005
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatacta
gatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggtt
ttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtact
acatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacat
gctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttc
gctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttact
ccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctgga
cgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacc
cagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctgg
ttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcga
ggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggc
gagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggc
ccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttctt
caagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgcc
gaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcc
tggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggt
gaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggc
gacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcg
atcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata