LOCUS       BBa_K574006             2517 bp    DNA     linear       22-Nov-2024
DEFINITION  3OC12HSL regulated by pTet and pBad
ACCESSION   BBa_K574006
VERSION     BBa_K574006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..686
                     /label=LasI
     misc_feature    687..719
                     /label=LVA
     misc_feature    726..750
                     /label=Help:Barcodes
     CDS             84..750
                     /label=BBa_C0078
     misc_feature    759..797
                     /label=B1006
     stem_loop       768..789
                     /label=modified thr terminator
     polyA_site      790..797
                     /label=PolyA
     polyA_site      759..767
                     /label=PolyA
     terminator      759..797
                     /label=BBa_B1006
     misc_feature    63..797
                     /label=BBa_K081016
     misc_feature    1..797
                     /label=BBa_K574004
     promoter        806..935
                     /label=BBa_I13453
     RBS             944..958
                     /label=RBS-1\Strong
     RBS             951..955
                     /label=RBS
     misc_feature    944..958
                     /label=BBa_B0030
     RBS             944..958
                     /label=BBa_B0030
     CDS             965..1567
                     /label=LasI
     misc_feature    1568..1600
                     /label=LVA
     misc_feature    1607..1631
                     /label=Help:Barcodes
     CDS             965..1631
                     /label=BBa_C0078
     misc_feature    944..1631
                     /label=BBa_K081009
     misc_feature    1644..1647
                     /label=conserved
     RBS             1640..1651
                     /label=BBa_B0034
     CDS             1658..2380
                     /label=BBa_E0030
     stem_loop       2400..2443
                     /label=stem_loop
     misc_feature    2389..2468
                     /label=BBa_B0010
     terminator      2389..2468
                     /label=BBa_B0010
     stem_loop       2484..2503
                     /label=T7 TE
     misc_feature    2510..2510
                     /label=stop
     polyA_site      2504..2517
                     /label=polya
     misc_feature    2477..2517
                     /label=BBa_B0012
     terminator      2477..2517
                     /label=BBa_B0012
     misc_feature    1640..2517
                     /label=BBa_E0430
     misc_feature    806..2517
                     /label=BBa_K574005
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca
      121 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt
      181 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact
      241 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg
      301 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag
      361 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag
      421 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct
      481 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga
      541 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg
      601 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc
      661 tggttgaaca gcgtctggct gtttccgctg ctaacgacga aaactacgct ctggttgctt
      721 aataactctg atagtgctag tgtagatctc tactagagaa aaaaaaaccc cgcccctgac
      781 agggcggggt ttttttttac tagagacatt gattatttgc acggcgtcac actttgctat
      841 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
      901 tctactgttt ctccataccg tttttttggg ctagctacta gagattaaag aggagaaata
      961 ctagatgatc gttcagatcg gtcgtcgtga agagttcgac aaaaaactgc tgggtgaaat
     1021 gcacaaactg cgtgctcagg ttttcaaaga acgtaaaggt tgggacgttt ccgttatcga
     1081 cgaaatggaa atcgacggtt acgacgctct gtccccgtac tacatgctga tccaggaaga
     1141 caccccggaa gctcaggttt tcggttgctg gcgtatcttc gacaccaccg gtccgtacat
     1201 gctgaaaaac accttcccgg aactgctgca cggtaaagaa gctccgtgct ccccgcacat
     1261 ctgggaactg tcccgtttcg ctatcaactc cggtcagaaa ggttccctgg gtttctccga
     1321 ctgcaccctg gaagctatgc gtgctctggc tcgttactcc ttgcagaacg acatccagac
     1381 cctggttacc gttaccaccg ttggtgttga aaaaatgatg atccgtgctg gtctggacgt
     1441 ttcccgtttc ggtccgcacc tgaaaatcgg tatcgaacgt gctgttgctc tgcgtatcga
     1501 actgaacgct aaaacccaga tcgctctgta cggtggtgtt ctggttgaac agcgtctggc
     1561 tgtttccgct gctaacgacg aaaactacgc tctggttgct taataactct gatagtgcta
     1621 gtgtagatct ctactagaga aagaggagaa atactagatg gtgagcaagg gcgaggagct
     1681 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1741 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1801 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     1861 cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct tcaagtccgc
     1921 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1981 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2041 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2101 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2161 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2221 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     2281 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2341 cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa
     2401 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2461 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
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