##gff-version 3
##sequence-region BBa_K574006 1 2517
BBa_K574006	.	engineered_region	1	797	.	+	0	ID=BBa_K574004;Name=BBa_K574004
BBa_K574006	.	engineered_region	63	797	.	+	0	ID=BBa_K081016;Name=BBa_K081016;Parent=BBa_K574004
BBa_K574006	.	terminator	759	797	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081016
BBa_K574006	.	polyA_site	759	767	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K574006	.	polyA_site	790	797	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K574006	.	stem_loop	768	789	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K574006	.	engineered_region	759	797	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K574006	.	CDS	84	750	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081016
BBa_K574006	.	tag	687	719	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K574006	.	engineered_tag	726	750	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K574006	.	CDS	84	686	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K574006	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081016
BBa_K574006	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K574006	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K574006	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K574006	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K574004
BBa_K574006	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K574006	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K574006	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K574006	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K574006	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K574006	.	engineered_region	806	2517	.	+	0	ID=BBa_K574005;Name=BBa_K574005
BBa_K574006	.	engineered_region	944	1631	.	+	0	ID=BBa_K081009;Name=BBa_K081009;Parent=BBa_K574005
BBa_K574006	.	CDS	965	1631	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_K081009
BBa_K574006	.	tag	1568	1600	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K574006	.	engineered_tag	1607	1631	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K574006	.	CDS	965	1567	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K574006	.	ribosome_entry_site	944	958	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081009
BBa_K574006	.	ribosome_entry_site	951	955	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K574006	.	engineered_region	944	958	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K574006	.	ribosome_entry_site	944	958	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K574006	.	promoter	806	935	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K574005
BBa_K574006	.	engineered_region	1640	2517	.	+	0	ID=BBa_E0430;Name=BBa_E0430;Parent=BBa_K574005
BBa_K574006	.	terminator	2389	2468	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_K574006	.	engineered_region	2389	2468	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K574006	.	stem_loop	2400	2443	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K574006	.	CDS	1658	2380	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_K574006	.	ribosome_entry_site	1640	1651	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_K574006	.	conserved_region	1644	1647	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K574006	.	terminator	2477	2517	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_K574006	.	stop_codon	2510	2510	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K574006	.	polyA_site	2504	2517	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K574006	.	stem_loop	2484	2503	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K574006	.	engineered_region	2477	2517	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K574006
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcag
gttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgt
actacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgta
catgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgt
ttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgtt
actccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtct
ggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaa
acccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctc
tggttgcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggt
ttttttttactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagc
ggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaa
agaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaa
ctgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacg
ctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacac
caccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgg
gaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtg
ctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgat
ccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaa
ctgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacg
aaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagat
ggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaag
ttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagc
tgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaa
gctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaac
tacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagg
aggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaa
gaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacc
ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta
caagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata