LOCUS       BBa_K574008              982 bp    DNA     linear       16-Feb-2025
DEFINITION  a constitutive LasR producer
ACCESSION   BBa_K574008
VERSION     BBa_K574008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    1..58
                     /label=BBa_K081005
     CDS             65..781
                     /label=lasR
     misc_feature    782..814
                     /label=LVA
     misc_feature    544..544
                     /label=G
     misc_feature    821..845
                     /label=Help:Barcodes
     CDS             65..845
                     /label=BBa_C0079
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
     terminator      854..982
                     /label=BBa_B0015
     misc_feature    65..982
                     /label=BBa_K574007
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatggcc ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg
      121 gagcgccatc ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct
      181 gttgcctaag gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc
      241 ctggcgcgag cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg
      301 tacccagagc gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca
      361 cgagttcttc gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca
      421 tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga
      481 ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact
      541 gcaaagcggt gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag
      601 ccgggagaag gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt
      661 tatctgcaac tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt
      721 cggtgtgacc tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct
      781 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag
      841 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta ta
//