##gff-version 3
##sequence-region BBa_K574009 1 1147
BBa_K574009	.	engineered_region	1	982	.	+	0	ID=BBa_K574008;Name=BBa_K574008
BBa_K574009	.	engineered_region	1	58	.	+	0	ID=BBa_K081005;Name=BBa_K081005;Parent=BBa_K574008
BBa_K574009	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K574009	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K574009	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K574009	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K574009	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K574009	.	engineered_region	65	982	.	+	0	ID=BBa_K574007;Name=BBa_K574007;Parent=BBa_K574008
BBa_K574009	.	CDS	65	845	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_K574007
BBa_K574009	.	CDS	65	781	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K574009	.	sequence_alteration	544	544	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K574009	.	engineered_tag	821	845	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K574009	.	tag	782	814	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K574009	.	terminator	854	982	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K574007
BBa_K574009	.	terminator	854	933	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K574009	.	engineered_region	854	933	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K574009	.	stem_loop	865	908	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K574009	.	terminator	942	982	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K574009	.	stem_loop	949	968	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K574009	.	engineered_region	942	982	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K574009	.	polyA_site	969	982	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K574009	.	stop_codon	975	975	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K574009	.	promoter	991	1147	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K574009	.	non_covalent_binding_site	1036	1053	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K574009	.	promoter	1130	1135	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K574009	.	non_covalent_binding_site	1096	1115	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K574009	.	promoter	1107	1112	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
>BBa_K574009
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccttggttgac
ggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggat
tctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggc
cgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactg
ccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctgg
tgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccg
ggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgcc
ggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgcca
tcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcg
gcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacc
tagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagtt
ctggcaggtttggccgcgggttctttttggtacacgaaagc