BBa_K574009
type: ComponentDefinition
persistent identity: BBa K574009
display id: BBa_K574009
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC12HSL -> PoPS Receiver
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: Existing Part.
owned by: james
owned by: myers
mutable description: PoPS -> LasI
discontinued: false
dominant: false
experience: experience/Works
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Empty.
star: false
creator: Lei Wei
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0079
component: BBa_K574008
sequence annotation: BBa_K574008
sequence annotation: BBa_R0079
sequence: https://synbiohub.org/public/igem/BBa_K574009_sequence/1

BBa_R0079
type: Component
persistent identity: component2264357
display id: component2264357
version: 1
top level: BBa_K574009
definition: LasR+PAI
access: public

BBa_K574008
type: Component
persistent identity: component2264352
display id: component2264352
version: 1
top level: BBa_K574009
definition: BBa_K574008
access: public

BBa_K574008
type: SequenceAnnotation
persistent identity: annotation2264352
display id: annotation2264352
version: 1
top level: BBa_K574009
location: https://synbiohub.org/public/igem/BBa_K574009/annotation2264352/range2264352/1
component: BBa_K574008

https://synbiohub.org/public/igem/BBa_K574009/annotation2264352/range2264352/1
type: Range
persistent identity: range2264352
display id: range2264352
version: 1
top level: BBa_K574009
direction: direction/reverse
start: 1
end: 982
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2264357
display id: annotation2264357
version: 1
top level: BBa_K574009
location: https://synbiohub.org/public/igem/BBa_K574009/annotation2264357/range2264357/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K574009/annotation2264357/range2264357/1
type: Range
persistent identity: range2264357
display id: range2264357
version: 1
top level: BBa_K574009
direction: direction/reverse
start: 991
end: 1147
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (LasR & PAI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Binding region for LasR protein (positive regulation)
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
top level: LasR+PAI
direction: direction/forward
start: 46
end: 63
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
top level: LasR+PAI
direction: direction/forward
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
top level: LasR+PAI
direction: direction/forward
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
top level: LasR+PAI
direction: direction/forward
start: 140
end: 145
orientation: inline

BBa_K574008
type: ComponentDefinition
persistent identity: BBa K574008
display id: BBa_K574008
version: 1
was generated by: iGEM to SBOL conversion
description: a constitutive LasR producer
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: Existing Parts.
owned by: james
owned by: myers
mutable description: const lasR
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Empty.
star: false
creator: Lei Wei
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K574007
component: BBa_K081005
sequence annotation: BBa_K081005
sequence annotation: BBa_K574007
sequence: https://synbiohub.org/public/igem/BBa_K574008_sequence/1

BBa_K574007
type: Component
persistent identity: component2258938
display id: component2258938
version: 1
top level: BBa_K574008
definition: BBa_K574007
access: public

BBa_K081005
type: Component
persistent identity: component2258925
display id: component2258925
version: 1
top level: BBa_K574008
definition: BBa_K081005
access: public

BBa_K081005
type: SequenceAnnotation
persistent identity: annotation2258925
display id: annotation2258925
version: 1
top level: BBa_K574008
location: https://synbiohub.org/public/igem/BBa_K574008/annotation2258925/range2258925/1
component: BBa_K081005

https://synbiohub.org/public/igem/BBa_K574008/annotation2258925/range2258925/1
type: Range
persistent identity: range2258925
display id: range2258925
version: 1
top level: BBa_K574008
direction: direction/reverse
start: 1
end: 58
orientation: inline

BBa_K574007
type: SequenceAnnotation
persistent identity: annotation2258938
display id: annotation2258938
version: 1
top level: BBa_K574008
location: https://synbiohub.org/public/igem/BBa_K574008/annotation2258938/range2258938/1
component: BBa_K574007

https://synbiohub.org/public/igem/BBa_K574008/annotation2258938/range2258938/1
type: Range
persistent identity: range2258938
display id: range2258938
version: 1
top level: BBa_K574008
direction: direction/reverse
start: 65
end: 982
orientation: inline

BBa_K081005
type: ComponentDefinition
persistent identity: BBa K081005
display id: BBa_K081005
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member and RBS
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:34Z
mutable provenance: Promoter: John Anderson. RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
discontinued: false
dominant: true
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23100
component: BBa_B0030
sequence annotation: BBa_J23100
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081005_sequence/1

BBa_J23100
type: Component
persistent identity: component1981863
display id: component1981863
version: 1
top level: BBa_K081005
definition: BBa_J23100
access: public

BBa_B0030
type: Component
persistent identity: component1981865
display id: component1981865
version: 1
top level: BBa_K081005
definition: BBa_B0030
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1981863
display id: annotation1981863
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
type: Range
persistent identity: range1981863
display id: range1981863
version: 1
top level: BBa_K081005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981865
display id: annotation1981865
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
type: Range
persistent identity: range1981865
display id: range1981865
version: 1
top level: BBa_K081005
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_K574007
type: ComponentDefinition
persistent identity: BBa K574007
display id: BBa_K574007
version: 1
was generated by: iGEM to SBOL conversion
description: lasR + TT
created: 2011-09-28T11:00:00Z
modified: 2015-05-08T01:12:44Z
mutable provenance: Existing Parts.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: lasR + T.
discontinued: false
dominant: false
experience: experience/None
group u list: _745_
m user id: 0
owner id: 8529
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Empty.
star: false
creator: Lei Wei
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_C0079
component: BBa_B0015
sequence annotation: BBa_C0079
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K574007_sequence/1

BBa_C0079
type: Component
persistent identity: component2248074
display id: component2248074
version: 1
top level: BBa_K574007
definition: lasr
access: public

BBa_B0015
type: Component
persistent identity: component2248081
display id: component2248081
version: 1
top level: BBa_K574007
definition: BBa_B0015
access: public

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2248074
display id: annotation2248074
version: 1
top level: BBa_K574007
location: https://synbiohub.org/public/igem/BBa_K574007/annotation2248074/range2248074/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K574007/annotation2248074/range2248074/1
type: Range
persistent identity: range2248074
display id: range2248074
version: 1
top level: BBa_K574007
direction: direction/reverse
start: 1
end: 781
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2248081
display id: annotation2248081
version: 1
top level: BBa_K574007
location: https://synbiohub.org/public/igem/BBa_K574007/annotation2248081/range2248081/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K574007/annotation2248081/range2248081/1
type: Range
persistent identity: range2248081
display id: range2248081
version: 1
top level: BBa_K574007
direction: direction/reverse
start: 790
end: 918
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutated 480 from g to a to remove PstI site
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lasR
sequence annotation: G
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
top level: lasr
direction: direction/forward
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
top level: lasr
direction: direction/forward
start: 480
end: 480
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
top level: lasr
direction: direction/forward
start: 718
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
top level: lasr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K574009_sequence/1
type: Sequence
persistent identity: BBa K574009 sequence
display id: BBa_K574009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0079_sequence/1
type: Sequence
persistent identity: BBa R0079 sequence
display id: BBa_R0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K574008_sequence/1
type: Sequence
persistent identity: BBa K574008 sequence
display id: BBa_K574008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081005_sequence/1
type: Sequence
persistent identity: BBa K081005 sequence
display id: BBa_K081005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K574007_sequence/1
type: Sequence
persistent identity: BBa K574007 sequence
display id: BBa_K574007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0079_sequence/1
type: Sequence
persistent identity: BBa C0079 sequence
display id: BBa_C0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z