LOCUS BBa_K575024 1701 bp DNA linear 22-Nov-2024 DEFINITION LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR ACCESSION BBa_K575024 VERSION BBa_K575024.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 RBS 166..180 /label=RBS-1\Strong RBS 173..177 /label=RBS misc_feature 166..180 /label=BBa_B0030 RBS 166..180 /label=BBa_B0030 CDS 187..906 /label=GFP protein CDS 187..906 /label=BBa_E0040 misc_feature 1..906 /label=BBa_K575008 promoter 915..949 /label=BBa_J23119 RBS 958..972 /label=RBS-1\Strong RBS 965..969 /label=RBS misc_feature 958..972 /label=BBa_B0030 RBS 958..972 /label=BBa_B0030 CDS 979..1701 /label=BBa_C0179 misc_feature 915..1701 /label=BBa_K575004 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa 181 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta 241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca 301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca 361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg 421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata 481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc 541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga 601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag 661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta 721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac 781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg 841 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa 901 taataatact agagttgaca gctagctcag tcctaggtat aatgctagct actagagatt 961 aaagaggaga aatactagat ggccttggtt gacggttttc ttgagctgga acgctcaagt 1021 ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg attctcgaag 1081 atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt catcgtcggc 1141 aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg ggtcgacccg 1201 acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc catctaccag 1261 acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt gtatgggctg 1321 accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag cgtggaagcg 1381 gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct gtggatgctc 1441 aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt cagcaaaccg 1501 gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg caagaccagt 1561 tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca tatgggaaat 1621 attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc cgttaatttg 1681 ggtcttatta ctctctaata a //