LOCUS BBa_K575025 1698 bp DNA linear 22-Nov-2024 DEFINITION LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0034) + LasR ACCESSION BBa_K575025 VERSION BBa_K575025.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 RBS 166..180 /label=RBS-1\Strong RBS 173..177 /label=RBS misc_feature 166..180 /label=BBa_B0030 RBS 166..180 /label=BBa_B0030 CDS 187..906 /label=GFP protein CDS 187..906 /label=BBa_E0040 misc_feature 1..906 /label=BBa_K575008 promoter 915..949 /label=BBa_J23119 misc_feature 962..965 /label=conserved RBS 958..969 /label=BBa_B0034 CDS 976..1698 /label=BBa_C0179 misc_feature 915..1698 /label=BBa_K575005 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa 181 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta 241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca 301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca 361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg 421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata 481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc 541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga 601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag 661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta 721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac 781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg 841 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa 901 taataatact agagttgaca gctagctcag tcctaggtat aatgctagct actagagaaa 961 gaggagaaat actagatggc cttggttgac ggttttcttg agctggaacg ctcaagtgga 1021 aaattggagt ggagcgccat cctccagaag atggcgagcg accttggatt ctcgaagatc 1081 ctgttcggcc tgttgcctaa ggacagccag gactacgaga acgccttcat cgtcggcaac 1141 tacccggccg cctggcgcga gcattacgac cgggctggct acgcgcgggt cgacccgacg 1201 gtcagtcact gtacccagag cgtactgccg attttctggg aaccgtccat ctaccagacg 1261 cgaaagcagc acgagttctt cgaggaagcc tcggccgccg gcctggtgta tgggctgacc 1321 atgccgctgc atggtgctcg cggcgaactc ggcgcgctga gcctcagcgt ggaagcggaa 1381 aaccgggccg aggccaaccg tttcatagag tcggtcctgc cgaccctgtg gatgctcaag 1441 gactacgcac tgcaaagcgg tgccggactg gccttcgaac atccggtcag caaaccggtg 1501 gttctgacca gccgggagaa ggaagtgttg cagtggtgcg ccatcggcaa gaccagttgg 1561 gagatatcgg ttatctgcaa ctgctcggaa gccaatgtga acttccatat gggaaatatt 1621 cggcggaagt tcggtgtgac ctcccgccgc gtagcggcca ttatggccgt taatttgggt 1681 cttattactc tctaataa //