LOCUS       BBa_K575025             1698 bp    DNA     linear       22-Dec-2024
DEFINITION  LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive 
            Promoter + RBS (B0034) + LasR
ACCESSION   BBa_K575025
VERSION     BBa_K575025.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..180
                     /label=RBS-1\Strong
     RBS             173..177
                     /label=RBS
     misc_feature    166..180
                     /label=BBa_B0030
     RBS             166..180
                     /label=BBa_B0030
     CDS             187..906
                     /label=GFP protein
     CDS             187..906
                     /label=BBa_E0040
     misc_feature    1..906
                     /label=BBa_K575008
     promoter        915..949
                     /label=BBa_J23119
     misc_feature    962..965
                     /label=conserved
     RBS             958..969
                     /label=BBa_B0034
     CDS             976..1698
                     /label=BBa_C0179
     misc_feature    915..1698
                     /label=BBa_K575005
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa
      181 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      841 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      901 taataatact agagttgaca gctagctcag tcctaggtat aatgctagct actagagaaa
      961 gaggagaaat actagatggc cttggttgac ggttttcttg agctggaacg ctcaagtgga
     1021 aaattggagt ggagcgccat cctccagaag atggcgagcg accttggatt ctcgaagatc
     1081 ctgttcggcc tgttgcctaa ggacagccag gactacgaga acgccttcat cgtcggcaac
     1141 tacccggccg cctggcgcga gcattacgac cgggctggct acgcgcgggt cgacccgacg
     1201 gtcagtcact gtacccagag cgtactgccg attttctggg aaccgtccat ctaccagacg
     1261 cgaaagcagc acgagttctt cgaggaagcc tcggccgccg gcctggtgta tgggctgacc
     1321 atgccgctgc atggtgctcg cggcgaactc ggcgcgctga gcctcagcgt ggaagcggaa
     1381 aaccgggccg aggccaaccg tttcatagag tcggtcctgc cgaccctgtg gatgctcaag
     1441 gactacgcac tgcaaagcgg tgccggactg gccttcgaac atccggtcag caaaccggtg
     1501 gttctgacca gccgggagaa ggaagtgttg cagtggtgcg ccatcggcaa gaccagttgg
     1561 gagatatcgg ttatctgcaa ctgctcggaa gccaatgtga acttccatat gggaaatatt
     1621 cggcggaagt tcggtgtgac ctcccgccgc gtagcggcca ttatggccgt taatttgggt
     1681 cttattactc tctaataa
//