LOCUS       BBa_K575026             1687 bp    DNA     linear       29-May-2024
DEFINITION  LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive 
            Promoter + RBS (B0030) + LasR
ACCESSION   BBa_K575026
VERSION     BBa_K575026.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..180
                     /label=RBS-1\Strong
     RBS             173..177
                     /label=RBS
     misc_feature    166..180
                     /label=BBa_B0030
     RBS             166..180
                     /label=BBa_B0030
     CDS             187..861
                     /label=mrfp1
     misc_feature    868..892
                     /label=Help:Barcodes
     CDS             187..892
                     /label=BBa_E1010
     misc_feature    1..892
                     /label=BBa_K575010
     promoter        901..935
                     /label=BBa_J23119
     RBS             944..958
                     /label=RBS-1\Strong
     RBS             951..955
                     /label=RBS
     misc_feature    944..958
                     /label=BBa_B0030
     RBS             944..958
                     /label=BBa_B0030
     CDS             965..1687
                     /label=BBa_C0179
     misc_feature    901..1687
                     /label=BBa_K575004
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa
      181 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
      241 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
      301 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
      361 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
      421 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
      481 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
      541 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
      601 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
      661 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
      721 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
      781 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
      841 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
      901 ttgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata
      961 ctagatggcc ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg
     1021 gagcgccatc ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct
     1081 gttgcctaag gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc
     1141 ctggcgcgag cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg
     1201 tacccagagc gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca
     1261 cgagttcttc gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca
     1321 tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga
     1381 ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact
     1441 gcaaagcggt gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag
     1501 ccgggagaag gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt
     1561 tatctgcaac tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt
     1621 cggtgtgacc tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct
     1681 ctaataa
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