LOCUS BBa_K575026 1687 bp DNA linear 22-Dec-2024 DEFINITION LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0030) + LasR ACCESSION BBa_K575026 VERSION BBa_K575026.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 RBS 166..180 /label=RBS-1\Strong RBS 173..177 /label=RBS misc_feature 166..180 /label=BBa_B0030 RBS 166..180 /label=BBa_B0030 CDS 187..861 /label=mrfp1 misc_feature 868..892 /label=Help:Barcodes CDS 187..892 /label=BBa_E1010 misc_feature 1..892 /label=BBa_K575010 promoter 901..935 /label=BBa_J23119 RBS 944..958 /label=RBS-1\Strong RBS 951..955 /label=RBS misc_feature 944..958 /label=BBa_B0030 RBS 944..958 /label=BBa_B0030 CDS 965..1687 /label=BBa_C0179 misc_feature 901..1687 /label=BBa_K575004 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa 181 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg 241 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac 301 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg 361 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac 421 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac 481 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc 541 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa 601 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa 661 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa 721 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa 781 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa 841 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag 901 ttgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata 961 ctagatggcc ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg 1021 gagcgccatc ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct 1081 gttgcctaag gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc 1141 ctggcgcgag cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg 1201 tacccagagc gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca 1261 cgagttcttc gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca 1321 tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga 1381 ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact 1441 gcaaagcggt gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag 1501 ccgggagaag gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt 1561 tatctgcaac tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt 1621 cggtgtgacc tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct 1681 ctaataa //