LOCUS       BBa_K575027             1684 bp    DNA     linear       22-Dec-2024
DEFINITION  LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive 
            Promoter + RBS (B0034) + LasR
ACCESSION   BBa_K575027
VERSION     BBa_K575027.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..180
                     /label=RBS-1\Strong
     RBS             173..177
                     /label=RBS
     misc_feature    166..180
                     /label=BBa_B0030
     RBS             166..180
                     /label=BBa_B0030
     CDS             187..861
                     /label=mrfp1
     misc_feature    868..892
                     /label=Help:Barcodes
     CDS             187..892
                     /label=BBa_E1010
     misc_feature    1..892
                     /label=BBa_K575010
     promoter        901..935
                     /label=BBa_J23119
     misc_feature    948..951
                     /label=conserved
     RBS             944..955
                     /label=BBa_B0034
     CDS             962..1684
                     /label=BBa_C0179
     misc_feature    901..1684
                     /label=BBa_K575005
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa
      181 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
      241 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
      301 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
      361 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
      421 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
      481 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
      541 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
      601 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
      661 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
      721 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
      781 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
      841 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
      901 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
      961 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
     1021 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
     1081 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
     1141 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
     1201 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
     1261 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
     1321 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
     1381 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
     1441 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
     1501 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
     1561 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
     1621 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta
     1681 ataa
//