LOCUS BBa_K575028 1698 bp DNA linear 22-Dec-2024 DEFINITION LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0030) + LasR ACCESSION BBa_K575028 VERSION BBa_K575028.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 CDS 184..903 /label=GFP protein CDS 184..903 /label=BBa_E0040 misc_feature 1..903 /label=BBa_K575009 promoter 912..946 /label=BBa_J23119 RBS 955..969 /label=RBS-1\Strong RBS 962..966 /label=RBS misc_feature 955..969 /label=BBa_B0030 RBS 955..969 /label=BBa_B0030 CDS 976..1698 /label=BBa_C0179 misc_feature 912..1698 /label=BBa_K575004 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 241 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac 301 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca 361 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa 421 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt 481 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt 541 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac 601 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat 661 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca 721 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 781 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc 841 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa 901 taatactaga gttgacagct agctcagtcc taggtataat gctagctact agagattaaa 961 gaggagaaat actagatggc cttggttgac ggttttcttg agctggaacg ctcaagtgga 1021 aaattggagt ggagcgccat cctccagaag atggcgagcg accttggatt ctcgaagatc 1081 ctgttcggcc tgttgcctaa ggacagccag gactacgaga acgccttcat cgtcggcaac 1141 tacccggccg cctggcgcga gcattacgac cgggctggct acgcgcgggt cgacccgacg 1201 gtcagtcact gtacccagag cgtactgccg attttctggg aaccgtccat ctaccagacg 1261 cgaaagcagc acgagttctt cgaggaagcc tcggccgccg gcctggtgta tgggctgacc 1321 atgccgctgc atggtgctcg cggcgaactc ggcgcgctga gcctcagcgt ggaagcggaa 1381 aaccgggccg aggccaaccg tttcatagag tcggtcctgc cgaccctgtg gatgctcaag 1441 gactacgcac tgcaaagcgg tgccggactg gccttcgaac atccggtcag caaaccggtg 1501 gttctgacca gccgggagaa ggaagtgttg cagtggtgcg ccatcggcaa gaccagttgg 1561 gagatatcgg ttatctgcaa ctgctcggaa gccaatgtga acttccatat gggaaatatt 1621 cggcggaagt tcggtgtgac ctcccgccgc gtagcggcca ttatggccgt taatttgggt 1681 cttattactc tctaataa //