LOCUS       BBa_K575030             1684 bp    DNA     linear       22-Nov-2024
DEFINITION  LasR/PAI1 Inducible Promoter + RBS (B0034) + RFP, Constitutive 
            Promoter + RBS (B0030) + LasR
ACCESSION   BBa_K575030
VERSION     BBa_K575030.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     CDS             184..858
                     /label=mrfp1
     misc_feature    865..889
                     /label=Help:Barcodes
     CDS             184..889
                     /label=BBa_E1010
     misc_feature    1..889
                     /label=BBa_K575011
     promoter        898..932
                     /label=BBa_J23119
     RBS             941..955
                     /label=RBS-1\Strong
     RBS             948..952
                     /label=RBS
     misc_feature    941..955
                     /label=BBa_B0030
     RBS             941..955
                     /label=BBa_B0030
     CDS             962..1684
                     /label=BBa_C0179
     misc_feature    898..1684
                     /label=BBa_K575004
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      241 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      301 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      361 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      421 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      481 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      541 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      601 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      661 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      721 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      781 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      841 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagttg
      901 acagctagct cagtcctagg tataatgcta gctactagag attaaagagg agaaatacta
      961 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
     1021 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
     1081 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
     1141 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
     1201 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
     1261 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
     1321 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
     1381 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
     1441 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
     1501 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
     1561 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
     1621 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta
     1681 ataa
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