##gff-version 3
##sequence-region BBa_K575030 1 1684
BBa_K575030	.	engineered_region	1	889	.	+	0	ID=BBa_K575011;Name=BBa_K575011
BBa_K575030	.	ribosome_entry_site	166	177	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K575011
BBa_K575030	.	conserved_region	170	173	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K575030	.	promoter	1	157	.	+	0	ID=BBa_R0079;Name=BBa_R0079;Parent=BBa_K575011
BBa_K575030	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K575030	.	promoter	140	145	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K575030	.	promoter	117	122	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K575030	.	non_covalent_binding_site	106	125	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K575030	.	CDS	184	889	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575011
BBa_K575030	.	CDS	184	858	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K575030	.	engineered_tag	865	889	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K575030	.	engineered_region	898	1684	.	+	0	ID=BBa_K575004;Name=BBa_K575004
BBa_K575030	.	promoter	898	932	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K575004
BBa_K575030	.	CDS	962	1684	.	+	0	ID=BBa_C0179;Name=BBa_C0179;Parent=BBa_K575004
BBa_K575030	.	ribosome_entry_site	941	955	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575004
BBa_K575030	.	engineered_region	941	955	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K575030	.	ribosome_entry_site	948	952	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K575030	.	ribosome_entry_site	941	955	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K575030
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatg
gaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgcta
aactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagc
ttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatg
aacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaac
tgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtat
gtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaa
gttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacct
cccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctg
atagtgctagtgtagatcgctactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaaga
ggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatc
ctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgaga
acgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgac
ggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttc
ttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgc
tgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgct
caaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgg
gagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagcca
atgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaa
tttgggtcttattactctctaataa