LOCUS       BBa_K575034             1589 bp    DNA     linear       22-Nov-2024
DEFINITION  RhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP + Constitutive 
            Promoter + RBS (B0030) + RhlR
ACCESSION   BBa_K575034
VERSION     BBa_K575034.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..76
                     /label=RBS-1\Strong
     RBS             69..73
                     /label=RBS
     misc_feature    62..76
                     /label=BBa_B0030
     RBS             62..76
                     /label=BBa_B0030
     CDS             83..757
                     /label=mrfp1
     misc_feature    764..788
                     /label=Help:Barcodes
     CDS             83..788
                     /label=BBa_E1010
     misc_feature    1..788
                     /label=BBa_K575014
     promoter        797..831
                     /label=BBa_J23119
     RBS             840..854
                     /label=RBS-1\Strong
     RBS             847..851
                     /label=RBS
     misc_feature    840..854
                     /label=BBa_B0030
     RBS             840..854
                     /label=BBa_B0030
     CDS             861..1589
                     /label=BBa_C0171
     misc_feature    797..1589
                     /label=BBa_K575006
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gattaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg
      121 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      181 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      241 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      301 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      361 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      421 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      481 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      541 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      601 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      661 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      721 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
      781 tagatcgcta ctagagttga cagctagctc agtcctaggt ataatgctag ctactagaga
      841 ttaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt
      901 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg
      961 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca
     1021 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga
     1081 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg
     1141 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt
     1201 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc
     1261 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc
     1321 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc
     1381 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg
     1441 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact
     1501 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct
     1561 acgccgcggc gctgggtctc atctaataa
//