LOCUS       BBa_K575035             1586 bp    DNA     linear       22-Nov-2024
DEFINITION  RhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP + Constitutive 
            Promoter + RBS (B0034) + RhlR
ACCESSION   BBa_K575035
VERSION     BBa_K575035.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..76
                     /label=RBS-1\Strong
     RBS             69..73
                     /label=RBS
     misc_feature    62..76
                     /label=BBa_B0030
     RBS             62..76
                     /label=BBa_B0030
     CDS             83..757
                     /label=mrfp1
     misc_feature    764..788
                     /label=Help:Barcodes
     CDS             83..788
                     /label=BBa_E1010
     misc_feature    1..788
                     /label=BBa_K575014
     promoter        797..831
                     /label=BBa_J23119
     misc_feature    844..847
                     /label=conserved
     RBS             840..851
                     /label=BBa_B0034
     CDS             858..1586
                     /label=BBa_C0171
     misc_feature    797..1586
                     /label=BBa_K575007
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gattaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg
      121 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      181 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      241 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      301 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      361 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      421 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      481 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      541 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      601 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      661 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      721 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
      781 tagatcgcta ctagagttga cagctagctc agtcctaggt ataatgctag ctactagaga
      841 aagaggagaa atactagatg aggaatgacg gaggcttttt gctgtggtgg gacggtttgc
      901 gtagcgagat gcagccgatc cacgacagcc agggcgtgtt cgccgtcctg gaaaaggaag
      961 tgcggcgcct gggcttcgat tactacgcct atggcgtgcg ccacacgatt cccttcaccc
     1021 ggccgaagac cgaggtccat ggcacctatc ccaaggcctg gctggagcga taccagatgc
     1081 agaactacgg ggccgtggat ccggcgatcc tcaacggcct gcgctcctcg gaaatggtgg
     1141 tctggagcga cagcctgttc gaccagagcc ggatgctctg gaacgaggct cgcgattggg
     1201 gcctctgtgt cggcgcgacc ttgccgatcc gcgcgccgaa caatttgctc agcgtgcttt
     1261 ccgtggcgcg cgaccagcag aacatctcca gcttcgagcg cgaggaaatc cgcctgcggc
     1321 tgcgttgcat gatcgagttg ctgacccaga agctgaccga cctggagcat ccgatgctga
     1381 tgtccaaccc ggtctgcctg agccatcgcg aacgcgagat cctgcaatgg accgccgacg
     1441 gcaagagttc cggggaaatc gccatcatcc tgagcatctc cgagagcacg gtgaacttcc
     1501 accacaagaa catccagaag aagttcgacg cgccgaacaa gacgctggct gccgcctacg
     1561 ccgcggcgct gggtctcatc taataa
//