##gff-version 3
##sequence-region BBa_K575035 1 1586
BBa_K575035	.	engineered_region	797	1586	.	+	0	ID=BBa_K575007;Name=BBa_K575007
BBa_K575035	.	ribosome_entry_site	840	851	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K575007
BBa_K575035	.	conserved_region	844	847	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K575035	.	CDS	858	1586	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_K575007
BBa_K575035	.	promoter	797	831	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K575007
BBa_K575035	.	engineered_region	1	788	.	+	0	ID=BBa_K575014;Name=BBa_K575014
BBa_K575035	.	promoter	1	53	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K575014
BBa_K575035	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K575035	.	start_codon	53	53	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K575035	.	promoter	19	24	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K575035	.	promoter	42	47	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K575035	.	ribosome_entry_site	62	76	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575014
BBa_K575035	.	ribosome_entry_site	69	73	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K575035	.	engineered_region	62	76	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K575035	.	ribosome_entry_site	62	76	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K575035	.	CDS	83	788	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575014
BBa_K575035	.	engineered_tag	764	788	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K575035	.	CDS	83	757	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K575035
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatac
tagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacg
agttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtgg
tccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgac
atcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttg
ttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtc
cgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctg
aaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggcta
aaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccat
cgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcta
ctagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaggaatgac
ggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcc
tggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaa
gaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcg
atcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacg
aggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgt
ggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctg
acccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcc
tgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttcca
ccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatc
taataa