LOCUS       BBa_K575038             1586 bp    DNA     linear       29-May-2024
DEFINITION  RhlR/PAI2 Inducible Promoter + RBS (B0034) + RFP + Constitutive 
            Promoter + RBS (B0030) + RhlR
ACCESSION   BBa_K575038
VERSION     BBa_K575038.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     misc_feature    66..69
                     /label=conserved
     RBS             62..73
                     /label=BBa_B0034
     CDS             80..754
                     /label=mrfp1
     misc_feature    761..785
                     /label=Help:Barcodes
     CDS             80..785
                     /label=BBa_E1010
     misc_feature    1..785
                     /label=BBa_K575015
     promoter        794..828
                     /label=BBa_J23119
     RBS             837..851
                     /label=RBS-1\Strong
     RBS             844..848
                     /label=RBS
     misc_feature    837..851
                     /label=BBa_B0030
     RBS             837..851
                     /label=BBa_B0030
     CDS             858..1586
                     /label=BBa_C0171
     misc_feature    794..1586
                     /label=BBa_K575006
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      121 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      181 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      241 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      301 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      361 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      421 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      481 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      541 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      601 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
      661 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
      721 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
      781 atcgctacta gagttgacag ctagctcagt cctaggtata atgctagcta ctagagatta
      841 aagaggagaa atactagatg aggaatgacg gaggcttttt gctgtggtgg gacggtttgc
      901 gtagcgagat gcagccgatc cacgacagcc agggcgtgtt cgccgtcctg gaaaaggaag
      961 tgcggcgcct gggcttcgat tactacgcct atggcgtgcg ccacacgatt cccttcaccc
     1021 ggccgaagac cgaggtccat ggcacctatc ccaaggcctg gctggagcga taccagatgc
     1081 agaactacgg ggccgtggat ccggcgatcc tcaacggcct gcgctcctcg gaaatggtgg
     1141 tctggagcga cagcctgttc gaccagagcc ggatgctctg gaacgaggct cgcgattggg
     1201 gcctctgtgt cggcgcgacc ttgccgatcc gcgcgccgaa caatttgctc agcgtgcttt
     1261 ccgtggcgcg cgaccagcag aacatctcca gcttcgagcg cgaggaaatc cgcctgcggc
     1321 tgcgttgcat gatcgagttg ctgacccaga agctgaccga cctggagcat ccgatgctga
     1381 tgtccaaccc ggtctgcctg agccatcgcg aacgcgagat cctgcaatgg accgccgacg
     1441 gcaagagttc cggggaaatc gccatcatcc tgagcatctc cgagagcacg gtgaacttcc
     1501 accacaagaa catccagaag aagttcgacg cgccgaacaa gacgctggct gccgcctacg
     1561 ccgcggcgct gggtctcatc taataa
//