BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K575041 1 BBa_K575041 Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP 2011-09-21T11:00:00Z 2015-05-08T01:12:45Z from genome and parts registry Genomic Promoter with GFP reporter false false _746_ 0 8735 9 It's complicated false no false Kristin Palarz component2138509 1 BBa_E0040 component2138505 1 BBa_K575021 component2138507 1 BBa_B0034 annotation2138507 1 BBa_B0034 range2138507 1 529 540 annotation2138505 1 BBa_K575021 range2138505 1 1 520 annotation2138509 1 BBa_E0040 range2138509 1 547 1266 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K575021 1 BBa_K575021 Genomic LasR/PAI1 Inducible Promoter (Short) 2011-09-21T11:00:00Z 2015-05-08T01:12:45Z It is the upstream regulatory region of [gene]. Released HQ 2013 This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-1/LasR complex, part of the Pseudomonas quorum sensing hierarchy. false false _746_ 0 8753 9 In stock false The part is unusually long for a promoter because of the primers we used to extract it (it is called short because it is shorter than companion part BBa_K575020. We do not know exactly where the regulatory region is. false Michael Vincent Sherer BBa_B0034_sequence 1 aaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K575041_sequence 1 ggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K575021_sequence 1 ggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z