BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K575043
1
BBa_K575043
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
from genome and registry
Genomic Promoter with GFP reporter
false
false
_746_
0
8735
9
It's complicated
false
no
false
Kristin Palarz
component2138517
1
BBa_B0030
component2138520
1
BBa_E0040
component2138515
1
BBa_K575023
annotation2138520
1
BBa_E0040
range2138520
1
551
1270
annotation2138515
1
BBa_K575023
range2138515
1
1
521
annotation2138517
1
BBa_B0030
range2138517
1
530
544
BBa_K575023
1
BBa_K575023
Genomic RhlR/PAI2 Inducible Promoter (short)
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
It is the upstream regulatory regions of (gene).
This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-2/RhlR complex, part of the Pseudomonas quorum sensing hierarchy.
false
false
_746_
0
8746
9
It's complicated
false
This part is unusually long for a promoter because of the primers we used to extract it. We do not know exactly where the regulatory region is.
false
Michael Vincent Sherer
BBa_K575023_sequence
1
aacagactttcgcgccgcatttcacacctcccaaaaattttcgaacaggcaaacagctatcgctgccacgggtatcccggcattacgtagagttcgttcttattgttcgaacggcagacaagtaactcagcggccatccgcgcggaccagccacggcggatgcgtaccccttcaggcgaggggcttgtgtgggtcttgcagatcggcctgcgcaaacgattggcgtccgtgttcacgcgtagccgatgaacactttttagccaattcgaaagctaacggtaactgccagatttcacaggacgggcggccgcctgcgcataagcccctgcccgcccgcgataaggcgtgccagagcggccggcggggaacagcgaggggttggcggaaaaggcatgaaaaaaccgggcgccaggcccggtttccggtgtcgaaacgctctggcgtcaggcttttggcagggtcacgccgcgctggccctggtatttgccgccacggtccttataggacacttcgcaggcctc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K575043_sequence
1
aacagactttcgcgccgcatttcacacctcccaaaaattttcgaacaggcaaacagctatcgctgccacgggtatcccggcattacgtagagttcgttcttattgttcgaacggcagacaagtaactcagcggccatccgcgcggaccagccacggcggatgcgtaccccttcaggcgaggggcttgtgtgggtcttgcagatcggcctgcgcaaacgattggcgtccgtgttcacgcgtagccgatgaacactttttagccaattcgaaagctaacggtaactgccagatttcacaggacgggcggccgcctgcgcataagcccctgcccgcccgcgataaggcgtgccagagcggccggcggggaacagcgaggggttggcggaaaaggcatgaaaaaaccgggcgccaggcccggtttccggtgtcgaaacgctctggcgtcaggcttttggcagggtcacgccgcgctggccctggtatttgccgccacggtccttataggacacttcgcaggcctctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z