BBa_K575044 1 BBa_K575044 Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR 2011-09-21T11:00:00Z 2015-05-08T01:12:45Z form genome and parts registry Released HQ 2013 Genomic LasP. producing GFP along with constiutivley producing LasR false false _746_ 0 8735 9 In stock false no false Kristin Palarz component2138532 1 BBa_K575004 component2138526 1 BBa_K575040 annotation2138532 1 BBa_K575004 range2138532 1 1556 2342 annotation2138526 1 BBa_K575040 range2138526 1 1 1547 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K575040 1 BBa_K575040 Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP 2011-09-21T11:00:00Z 2015-05-08T01:12:45Z Genome and registry Released HQ 2013 Genomic promoter with GFP reporter false false _746_ 0 8735 9 In stock false no false Kristin Palarz component2138500 1 BBa_K575020 component2138502 1 BBa_B0034 component2138504 1 BBa_E0040 annotation2138502 1 BBa_B0034 range2138502 1 810 821 annotation2138504 1 BBa_E0040 range2138504 1 828 1547 annotation2138500 1 BBa_K575020 range2138500 1 1 801 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_C0179 1 lasR lasR activator from P. aeruginosa PAO1(no LVA) 2004-05-26T11:00:00Z 2015-08-31T04:07:24Z Released HQ 2013 same as C0079 except no LVA tag false false _11_1_ 0 61 7 In stock false true jcbraff BBa_K575020 1 BBa_K575020 Genomic LasR/PAI1 Inducible Promoter (Long) 2011-09-21T11:00:00Z 2015-05-08T01:12:45Z It is the upstream regulatory region of [gene]. Released HQ 2013 This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-1/LasR complex, part of the Pseudomonas quorum sensing hierarchy. false false _746_ 0 8753 9 In stock false The part is unusually long for a promoter because of the primers we used to extract it. We do not know exactly where the regulatory region is. false Michael Vincent Sherer BBa_K575004 1 BBa_K575004 Continuous expression of LasR receptor protein (with RBS, B0030) 2011-08-10T11:00:00Z 2015-05-08T01:12:44Z Composite part consisting of individual parts in the registry. With RBS B0030. false false _746_ 0 8746 9 It's complicated false Optimal strength of constitutive promoter. false Nirmit Desai component2124800 1 BBa_B0030 component2124798 1 BBa_J23119 component2124802 1 BBa_C0179 annotation2124800 1 BBa_B0030 range2124800 1 44 58 annotation2124802 1 BBa_C0179 range2124802 1 65 787 annotation2124798 1 BBa_J23119 range2124798 1 1 35 BBa_B0034_sequence 1 aaagaggagaaa BBa_C0179_sequence 1 atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa BBa_K575020_sequence 1 ccctctctctcatcatgcatgccagcttcaccagtcaacaatcgatcccggcaggattcggttcttcagcgtttttattcttttttgtttccgtcttatccactcatgacaattccctttatcaagccggacctggaagtacatatcgccaccggccctgtgcgggtgttccggacgaggtcgagcccattcgccgcccgccgggcgacgacgaaacgaggctgaagagaacttctcgatgagcacggacacgaggcgccgcgcaggaactgcgctgggctggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcgg BBa_K575040_sequence 1 ccctctctctcatcatgcatgccagcttcaccagtcaacaatcgatcccggcaggattcggttcttcagcgtttttattcttttttgtttccgtcttatccactcatgacaattccctttatcaagccggacctggaagtacatatcgccaccggccctgtgcgggtgttccggacgaggtcgagcccattcgccgcccgccgggcgacgacgaaacgaggctgaagagaacttctcgatgagcacggacacgaggcgccgcgcaggaactgcgctgggctggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0030_sequence 1 attaaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K575004_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa BBa_K575044_sequence 1 ccctctctctcatcatgcatgccagcttcaccagtcaacaatcgatcccggcaggattcggttcttcagcgtttttattcttttttgtttccgtcttatccactcatgacaattccctttatcaagccggacctggaagtacatatcgccaccggccctgtgcgggtgttccggacgaggtcgagcccattcgccgcccgccgggcgacgacgaaacgaggctgaagagaacttctcgatgagcacggacacgaggcgccgcgcaggaactgcgctgggctggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z