BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K575004
1
BBa_K575004
Continuous expression of LasR receptor protein (with RBS, B0030)
2011-08-10T11:00:00Z
2015-05-08T01:12:44Z
Composite part consisting of individual parts in the registry.
With RBS B0030.
false
false
_746_
0
8746
9
It's complicated
false
Optimal strength of constitutive promoter.
false
Nirmit Desai
component2124802
1
BBa_C0179
component2124798
1
BBa_J23119
component2124800
1
BBa_B0030
annotation2124800
1
BBa_B0030
range2124800
1
44
58
annotation2124798
1
BBa_J23119
range2124798
1
1
35
annotation2124802
1
BBa_C0179
range2124802
1
65
787
BBa_K575045
1
BBa_K575045
Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0030) + LasR
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
from genome and registry
Released HQ 2013
Genomic LasP. producing GFP along with constiutivley producing LasR
false
false
_746_
0
8735
9
In stock
false
no
false
Kristin Palarz
component2138539
1
BBa_K575041
component2138545
1
BBa_K575004
annotation2138539
1
BBa_K575041
range2138539
1
1
1266
annotation2138545
1
BBa_K575004
range2138545
1
1275
2061
BBa_K575041
1
BBa_K575041
Genomic LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
from genome and parts registry
Genomic Promoter with GFP reporter
false
false
_746_
0
8735
9
It's complicated
false
no
false
Kristin Palarz
component2138507
1
BBa_B0034
component2138505
1
BBa_K575021
component2138509
1
BBa_E0040
annotation2138509
1
BBa_E0040
range2138509
1
547
1266
annotation2138507
1
BBa_B0034
range2138507
1
529
540
annotation2138505
1
BBa_K575021
range2138505
1
1
520
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_C0179
1
lasR
lasR activator from P. aeruginosa PAO1(no LVA)
2004-05-26T11:00:00Z
2015-08-31T04:07:24Z
Released HQ 2013
same as C0079 except no LVA tag
false
false
_11_1_
0
61
7
In stock
false
true
jcbraff
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K575021
1
BBa_K575021
Genomic LasR/PAI1 Inducible Promoter (Short)
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
It is the upstream regulatory region of [gene].
Released HQ 2013
This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-1/LasR complex, part of the Pseudomonas quorum sensing hierarchy.
false
false
_746_
0
8753
9
In stock
false
The part is unusually long for a promoter because of the primers we used to extract it (it is called short because it is shorter than companion part BBa_K575020. We do not know exactly where the regulatory region is.
false
Michael Vincent Sherer
BBa_B0034_sequence
1
aaagaggagaaa
BBa_C0179_sequence
1
atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
BBa_K575045_sequence
1
ggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K575004_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_K575041_sequence
1
ggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K575021_sequence
1
ggaggggacggcgccccggatctttgcggaaaccgtagaacggctctccgatgggcctcacggcggtctttctcattgttcttctcgcacgctccatcgtcgtcgggagagcctcccgacaacaaaccttggcccatggcgggcctcgtcgacgaggctccccggggaccagaaatggcaactacatacttccccccctatctctccgcagatacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattgtccgtgccgattttcacgaagcgcattctgaggcaattaaaaagagcgctccattcgaccatggacaagctatccacgcctgaccgagatcgccttccgaatatagcgaagcgataaccgcagcctgccgagaagtgcttcagacaataaacaggacgctggcctttcgtatcgatgaaagttccgcatggcgtccgcccctaaggaagaggagataaatatgatttattacttgatcgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z