BBa_K575046
1
BBa_K575046
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
from genome and registry
Genomic RhlP. producing GFP along with constiutivley producing RhlR
false
false
_746_
0
8735
9
It's complicated
true
no
false
Kristin Palarz
component2138556
1
BBa_K575007
component2138551
1
BBa_K575042
annotation2138551
1
BBa_K575042
range2138551
1
1
1267
annotation2138556
1
BBa_K575007
range2138556
1
1276
2065
BBa_K575042
1
BBa_K575042
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
from genome and parts registry
Genomic Promoter with GFP reporter
false
false
_746_
0
8735
9
It's complicated
false
no
false
Kristin Palarz
component2138514
1
BBa_E0040
component2138512
1
BBa_B0034
component2138510
1
BBa_K575023
annotation2138510
1
BBa_K575023
range2138510
1
1
521
annotation2138512
1
BBa_B0034
range2138512
1
530
541
annotation2138514
1
BBa_E0040
range2138514
1
548
1267
BBa_C0171
1
rhIR
rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
2004-05-26T11:00:00Z
2015-08-31T04:07:24Z
Released HQ 2013
same as C0071 except no LVA tag
false
false
_11_1_
0
61
7
In stock
false
true
jcbraff
BBa_K575007
1
BBa_K575007
Continous expression of RhlR receptor protein (with RBS, B0034)
2011-08-10T11:00:00Z
2015-05-08T01:12:45Z
These parts come from the BioBrick Registry
This is a Constitutive Promoter combined with RBS (B0034) and RhlR so that the cell continuously expresses RhlR.
false
false
_746_
0
8735
9
It's complicated
false
The strength of the Constitutive promoter.
false
Kristin Palarz
component2124807
1
BBa_C0171
component2124806
1
BBa_B0034
component2124804
1
BBa_J23119
annotation2124806
1
BBa_B0034
range2124806
1
44
55
annotation2124807
1
BBa_C0171
range2124807
1
62
790
annotation2124804
1
BBa_J23119
range2124804
1
1
35
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K575023
1
BBa_K575023
Genomic RhlR/PAI2 Inducible Promoter (short)
2011-09-21T11:00:00Z
2015-05-08T01:12:45Z
It is the upstream regulatory regions of (gene).
This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the PAI-2/RhlR complex, part of the Pseudomonas quorum sensing hierarchy.
false
false
_746_
0
8746
9
It's complicated
false
This part is unusually long for a promoter because of the primers we used to extract it. We do not know exactly where the regulatory region is.
false
Michael Vincent Sherer
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K575023_sequence
1
aacagactttcgcgccgcatttcacacctcccaaaaattttcgaacaggcaaacagctatcgctgccacgggtatcccggcattacgtagagttcgttcttattgttcgaacggcagacaagtaactcagcggccatccgcgcggaccagccacggcggatgcgtaccccttcaggcgaggggcttgtgtgggtcttgcagatcggcctgcgcaaacgattggcgtccgtgttcacgcgtagccgatgaacactttttagccaattcgaaagctaacggtaactgccagatttcacaggacgggcggccgcctgcgcataagcccctgcccgcccgcgataaggcgtgccagagcggccggcggggaacagcgaggggttggcggaaaaggcatgaaaaaaccgggcgccaggcccggtttccggtgtcgaaacgctctggcgtcaggcttttggcagggtcacgccgcgctggccctggtatttgccgccacggtccttataggacacttcgcaggcctc
BBa_K575007_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K575042_sequence
1
aacagactttcgcgccgcatttcacacctcccaaaaattttcgaacaggcaaacagctatcgctgccacgggtatcccggcattacgtagagttcgttcttattgttcgaacggcagacaagtaactcagcggccatccgcgcggaccagccacggcggatgcgtaccccttcaggcgaggggcttgtgtgggtcttgcagatcggcctgcgcaaacgattggcgtccgtgttcacgcgtagccgatgaacactttttagccaattcgaaagctaacggtaactgccagatttcacaggacgggcggccgcctgcgcataagcccctgcccgcccgcgataaggcgtgccagagcggccggcggggaacagcgaggggttggcggaaaaggcatgaaaaaaccgggcgccaggcccggtttccggtgtcgaaacgctctggcgtcaggcttttggcagggtcacgccgcgctggccctggtatttgccgccacggtccttataggacacttcgcaggcctctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_C0171_sequence
1
atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
BBa_K575046_sequence
1
aacagactttcgcgccgcatttcacacctcccaaaaattttcgaacaggcaaacagctatcgctgccacgggtatcccggcattacgtagagttcgttcttattgttcgaacggcagacaagtaactcagcggccatccgcgcggaccagccacggcggatgcgtaccccttcaggcgaggggcttgtgtgggtcttgcagatcggcctgcgcaaacgattggcgtccgtgttcacgcgtagccgatgaacactttttagccaattcgaaagctaacggtaactgccagatttcacaggacgggcggccgcctgcgcataagcccctgcccgcccgcgataaggcgtgccagagcggccggcggggaacagcgaggggttggcggaaaaggcatgaaaaaaccgggcgccaggcccggtttccggtgtcgaaacgctctggcgtcaggcttttggcagggtcacgccgcgctggccctggtatttgccgccacggtccttataggacacttcgcaggcctctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z