LOCUS       BBa_K577881             2136 bp    DNA     linear       22-Nov-2024
DEFINITION  pC+AraC+pBad+GFPc
ACCESSION   BBa_K577881
VERSION     BBa_K577881.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1114
                     /label=BBa_I13458
     promoter        1123..1252
                     /label=BBa_I13453
     RBS             1261..1273
                     /label=RBS-3\Weak
     RBS             1267..1270
                     /label=RBS
     misc_feature    1261..1273
                     /label=BBa_B0032
     RBS             1261..1273
                     /label=BBa_B0032
     CDS             1280..1999
                     /label=GFP protein
     CDS             1280..1999
                     /label=BBa_E0040
     stem_loop       2019..2062
                     /label=stem_loop
     misc_feature    2008..2087
                     /label=BBa_B0010
     terminator      2008..2087
                     /label=BBa_B0010
     stem_loop       2103..2122
                     /label=T7 TE
     misc_feature    2129..2129
                     /label=stop
     polyA_site      2123..2136
                     /label=polya
     misc_feature    2096..2136
                     /label=BBa_B0012
     terminator      2096..2136
                     /label=BBa_B0012
     misc_feature    1261..2136
                     /label=BBa_E0240
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg
     1141 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag
     1261 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
     1321 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1381 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1441 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1501 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1561 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1621 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1681 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1741 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1801 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1861 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1921 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1981 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2041 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2101 ctggctcacc ttcgggtggg cctttctgcg tttata
//