LOCUS       BBa_K577883              929 bp    DNA     linear       22-Nov-2024
DEFINITION  pC+GFPc
ACCESSION   BBa_K577883
VERSION     BBa_K577883.1  
FEATURES             Location/Qualifiers
     promoter        10..15
                     /label=-35
     promoter        33..38
                     /label=-10
     misc_feature    20..33
                     /label=Zif23
     misc_feature    1..9
                     /label=consensus
     misc_feature    45..45
                     /label=(+1)
     promoter        1..45
                     /label=BBa_R2000
     RBS             54..66
                     /label=RBS-3\Weak
     RBS             60..63
                     /label=RBS
     misc_feature    54..66
                     /label=BBa_B0032
     RBS             54..66
                     /label=BBa_B0032
     CDS             73..792
                     /label=GFP protein
     CDS             73..792
                     /label=BBa_E0040
     stem_loop       812..855
                     /label=stem_loop
     misc_feature    801..880
                     /label=BBa_B0010
     terminator      801..880
                     /label=BBa_B0010
     stem_loop       896..915
                     /label=T7 TE
     misc_feature    922..922
                     /label=stop
     polyA_site      916..929
                     /label=polya
     misc_feature    889..929
                     /label=BBa_B0012
     terminator      889..929
                     /label=BBa_B0012
     misc_feature    54..929
                     /label=BBa_E0240
ORIGIN
        1 agtttattct tgacatggtc ccacgcgcgt gggatactac gtcagtacta gagtcacaca
       61 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      121 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      181 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      241 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
      301 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      361 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      421 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      481 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      541 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
      601 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      661 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      721 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      781 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      901 accttcgggt gggcctttct gcgtttata
//