This part comes from ligating 2 biobricks.
This reporter part is a combination of a pBad promoter (inducible with arabinose) and a RFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible genetic device. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. But It is important for the user to determine if his or her cell line contain AraC gene, since AraC gene is required to actually induce pBad promoter with arabinose. See BBa_K577882 or BBa_I13458 for more information.
The RBS, RFP, and Terminator were taken from the BBa_J61002 backbone using SpeI and PstI restriction enzymes.
This is a construct for testing promoter strength by measuring RFP fluorescence.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
Double terminator consisting of BBa_B0010 and BBa_B0012
Built by Josh as an intermediate in screening plasmid construction.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1