LOCUS       BBa_K577893             1832 bp    DNA     linear       14-May-2024
DEFINITION  TetR repressed GFP
ACCESSION   BBa_K577893
VERSION     BBa_K577893.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             68..684
                     /label=tetR
     misc_feature    685..718
                     /label=SsrA
     misc_feature    725..749
                     /label=Help:Barcodes
     CDS             65..749
                     /label=BBa_C0040
     stem_loop       769..812
                     /label=stem_loop
     misc_feature    758..837
                     /label=BBa_B0010
     terminator      758..837
                     /label=BBa_B0010
     stem_loop       853..872
                     /label=T7 TE
     misc_feature    879..879
                     /label=stop
     polyA_site      873..886
                     /label=polya
     misc_feature    846..886
                     /label=BBa_B0012
     terminator      846..886
                     /label=BBa_B0012
     misc_feature    895..913
                     /label=TetR 1
     misc_feature    895..948
                     /label=BBa_R0040
     promoter        914..919
                     /label=-35
     misc_feature    920..938
                     /label=TetR 2
     promoter        937..942
                     /label=-10
     promoter        895..948
                     /label=BBa_R0040
     RBS             957..969
                     /label=RBS-3\Weak
     RBS             963..966
                     /label=RBS
     misc_feature    957..969
                     /label=BBa_B0032
     RBS             957..969
                     /label=BBa_B0032
     CDS             976..1695
                     /label=GFP protein
     CDS             976..1695
                     /label=BBa_E0040
     stem_loop       1715..1758
                     /label=stem_loop
     misc_feature    1704..1783
                     /label=BBa_B0010
     terminator      1704..1783
                     /label=BBa_B0010
     stem_loop       1799..1818
                     /label=T7 TE
     misc_feature    1825..1825
                     /label=stop
     polyA_site      1819..1832
                     /label=polya
     misc_feature    1792..1832
                     /label=BBa_B0012
     terminator      1792..1832
                     /label=BBa_B0012
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
      121 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
      181 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
      241 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
      301 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
      361 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
      421 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
      481 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
      541 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
      601 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
      661 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta
      721 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
      781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      841 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      901 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagtcac
      961 acaggaaagt actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt
     1021 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     1081 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1141 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca
     1201 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     1261 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     1321 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     1381 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     1441 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     1501 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     1561 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1621 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     1681 ctatacaaat aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1741 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1801 ctcaccttcg ggtgggcctt tctgcgttta ta
//