LOCUS       BBa_K577894             1834 bp    DNA     linear       14-May-2024
DEFINITION  TetR repressed YFP
ACCESSION   BBa_K577894
VERSION     BBa_K577894.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             68..684
                     /label=tetR
     misc_feature    685..718
                     /label=SsrA
     misc_feature    725..749
                     /label=Help:Barcodes
     CDS             65..749
                     /label=BBa_C0040
     stem_loop       769..812
                     /label=stem_loop
     misc_feature    758..837
                     /label=BBa_B0010
     terminator      758..837
                     /label=BBa_B0010
     stem_loop       853..872
                     /label=T7 TE
     misc_feature    879..879
                     /label=stop
     polyA_site      873..886
                     /label=polya
     misc_feature    846..886
                     /label=BBa_B0012
     terminator      846..886
                     /label=BBa_B0012
     misc_feature    895..913
                     /label=TetR 1
     misc_feature    895..948
                     /label=BBa_R0040
     promoter        914..919
                     /label=-35
     misc_feature    920..938
                     /label=TetR 2
     promoter        937..942
                     /label=-10
     promoter        895..948
                     /label=BBa_R0040
     misc_feature    961..964
                     /label=conserved
     RBS             957..968
                     /label=BBa_B0034
     CDS             975..1697
                     /label=BBa_E0030
     stem_loop       1717..1760
                     /label=stem_loop
     misc_feature    1706..1785
                     /label=BBa_B0010
     terminator      1706..1785
                     /label=BBa_B0010
     stem_loop       1801..1820
                     /label=T7 TE
     misc_feature    1827..1827
                     /label=stop
     polyA_site      1821..1834
                     /label=polya
     misc_feature    1794..1834
                     /label=BBa_B0012
     terminator      1794..1834
                     /label=BBa_B0012
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
      121 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
      181 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
      241 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
      301 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
      361 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
      421 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
      481 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
      541 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
      601 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
      661 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta
      721 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
      781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      841 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      901 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      961 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1021 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1081 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1141 tgccctggcc caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc
     1201 ccgaccacat gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1261 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1321 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1381 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1441 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1501 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1561 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     1621 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1681 agctgtacaa gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1741 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1801 ggctcacctt cgggtgggcc tttctgcgtt tata
//