LOCUS       BBa_K577895             1817 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR repressed RFP
ACCESSION   BBa_K577895
VERSION     BBa_K577895.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             68..684
                     /label=tetR
     misc_feature    685..718
                     /label=SsrA
     misc_feature    725..749
                     /label=Help:Barcodes
     CDS             65..749
                     /label=BBa_C0040
     stem_loop       769..812
                     /label=stem_loop
     misc_feature    758..837
                     /label=BBa_B0010
     terminator      758..837
                     /label=BBa_B0010
     stem_loop       853..872
                     /label=T7 TE
     misc_feature    879..879
                     /label=stop
     polyA_site      873..886
                     /label=polya
     misc_feature    846..886
                     /label=BBa_B0012
     terminator      846..886
                     /label=BBa_B0012
     misc_feature    895..913
                     /label=TetR 1
     misc_feature    895..948
                     /label=BBa_R0040
     promoter        914..919
                     /label=-35
     misc_feature    920..938
                     /label=TetR 2
     promoter        937..942
                     /label=-10
     promoter        895..948
                     /label=BBa_R0040
     misc_feature    961..964
                     /label=conserved
     RBS             957..968
                     /label=BBa_B0034
     CDS             975..1649
                     /label=mrfp1
     misc_feature    1656..1680
                     /label=Help:Barcodes
     CDS             975..1680
                     /label=BBa_E1010
     stem_loop       1700..1743
                     /label=stem_loop
     misc_feature    1689..1768
                     /label=BBa_B0010
     terminator      1689..1768
                     /label=BBa_B0010
     stem_loop       1784..1803
                     /label=T7 TE
     misc_feature    1810..1810
                     /label=stop
     polyA_site      1804..1817
                     /label=polya
     misc_feature    1777..1817
                     /label=BBa_B0012
     terminator      1777..1817
                     /label=BBa_B0012
     terminator      1689..1817
                     /label=BBa_B0015
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
      121 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
      181 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
      241 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
      301 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
      361 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
      421 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
      481 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
      541 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
      601 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
      661 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta
      721 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
      781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      841 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      901 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      961 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag
     1021 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc
     1081 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt
     1141 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc
     1201 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg
     1261 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg
     1321 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta
     1381 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg
     1441 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg
     1501 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg
     1561 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac
     1621 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc
     1681 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1741 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1801 gcctttctgc gtttata
//