LOCUS       BBa_K577896             1301 bp    DNA     linear       29-Nov-2024
DEFINITION  pTet promoted GFP
ACCESSION   BBa_K577896
VERSION     BBa_K577896.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..797
                     /label=GFP
     CDS             798..815
                     /label=linker
     misc_feature    816..1301
                     /label=PEST191 (degradation flag)
     misc_feature    81..1301
                     /label=GFP-linker-PEST191
     misc_feature    81..83
                     /label=start codon
     misc_feature    1299..1301
                     /label=stop codon
     misc_feature    81..1301
                     /label=BBa_J52028
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagggt ggttctggtg gtactgctgc ggtgctgacc ttggccgtgc
      841 tcttcctgac ggggagccag gctcggcatt tctggcagca agatgaaccc ccccagagcc
      901 cctgggatcg agtgaaggac ctggccactg tgtacgtgga tgtgctcaaa gacagcggca
      961 gagactatgt gtcccagttt gaaggctccg ccttgggaaa acagctaaac ctaaagctcc
     1021 ttgacaactg ggacagcgtg acctccacct tcagcaagct gcgcgaacag ctcggccctg
     1081 tgacccagga gttctgggat aacctggaaa aggagacaga gggcctgagg caggagatga
     1141 gcaaggatct ggaggaggtg aaggccaagg tgcagcccta cctggacgac ttccagaaga
     1201 agtggcagga ggagatggag ctctaccgcc agaaggtgga gccgctgcgc gcagagctcc
     1261 aagagggcgc gcgccagaag ctgcacgagc tgcaagagta g
//