##gff-version 3
##sequence-region BBa_K577896 1 1301
BBa_K577896	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K577896	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K577896	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K577896	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K577896	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K577896	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K577896	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K577896	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K577896	.	engineered_region	81	1301	.	+	0	ID=BBa_J52028;Name=BBa_J52028
BBa_K577896	.	CDS	798	815	.	+	0	ID=annotation1903015;Name=linker;Parent=BBa_J52028
BBa_K577896	.	tag	816	1301	.	+	0	ID=annotation1903016;Name=PEST191 (degradation flag);Parent=BBa_J52028
BBa_K577896	.	start_codon	81	83	.	+	0	ID=annotation1908104;Name=start codon;Parent=BBa_J52028
BBa_K577896	.	stop_codon	1299	1301	.	+	0	ID=annotation1908105;Name=stop codon;Parent=BBa_J52028
BBa_K577896	.	engineered_region	81	1301	.	+	0	ID=annotation1903017;Name=GFP-linker-PEST191;Parent=BBa_J52028
BBa_K577896	.	CDS	81	797	.	+	0	ID=annotation1903014;Name=GFP;Parent=BBa_J52028
>BBa_K577896
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcgcccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaacgccatcagcgacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagggtggttctggtggtactgctgcggtgctgaccttggccgtgctcttcctgacggggagccaggctcggcat
ttctggcagcaagatgaacccccccagagcccctgggatcgagtgaaggacctggccactgtgtacgtggatgtgctca
aagacagcggcagagactatgtgtcccagtttgaaggctccgccttgggaaaacagctaaacctaaagctccttgacaa
ctgggacagcgtgacctccaccttcagcaagctgcgcgaacagctcggccctgtgacccaggagttctgggataacctg
gaaaaggagacagagggcctgaggcaggagatgagcaaggatctggaggaggtgaaggccaaggtgcagccctacctgg
acgacttccagaagaagtggcaggaggagatggagctctaccgccagaaggtggagccgctgcgcgcagagctccaaga
gggcgcgcgccagaagctgcacgagctgcaagagtag