BBa_K579000
1
no ATG GFP
no ATG GFP
2011-10-04T11:00:00Z
2015-05-08T01:12:46Z
820base sequence
AAACCCATAATATCATGACATACCCTATAAAAATAGGCGTATCACGAGGCAGAATTCAGATAAAAAAAATCCCTAGCTTTCGCTAAGGATGATTCTGGAATTCTTTCTAGATTCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATTTTAATCT
This plasmid vector was designed to fuse GFP to C-terminal regions of any proteins.(We use BBa_E0240.)
In our projects, we use this plasmid to fuse GFP to dMLF, DIAP2, API2-MALT1 and others to monitor expression of these proteins in E. coli and Drosophila.
false
true
_750_
0
7778
9
In stock
true
Primer sequence
F:TTGAATTCTTTTCTAGATTTCGTAAAGGAGAAGAA
R:AAACTGCAGAAAACTAGTTTTTTATTATTTGTATAG
false
Reo Yoshimura,Toshihiro Matsunami,Takahiro Nakagawa,Jyunpei Yokoigawa
annotation2155666
1
BBa_K579000
range2155666
1
1
717
annotation2155667
1
no ATG GFP
range2155667
1
1
717
BBa_K579000_sequence
1
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z