##gff-version 3
##sequence-region BBa_K581000 174 364
BBa_K581000	.	point_mutation	178	178	.	+	0	ID=annotation2152330;Name=G178C mutant of SgrS(wt)
BBa_K581000	.	engineered_region	236	364	.	+	0	ID=annotation2152356;Name=B0015
BBa_K581000	.	conserved_region	174	179	.	+	0	ID=annotation2130113;Name=critical region of SgrS/ptsG interaction
>BBa_K581000
gatgaagcaagggggtgccccatgcgtcagttttatcagcactattttaccgcgacagcgaagttgtgctggttgcgtt
ggttaagcgtcccacaacgattaaccatgcttgaaggactgatgcagtgggatgaccgcaattctgaaagttgacttgc
ctgcatcatgtgtgactgactattggtgtaaaatcacccgccagcagattatacctgctggtttttttttactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata