LOCUS       BBa_K581001              960 bp    DNA     linear       26-Dec-2024
DEFINITION  ptsG1-GFP (ptsG1 5' UTR fused with gfp)
ACCESSION   BBa_K581001
VERSION     BBa_K581001.1  
FEATURES             Location/Qualifiers
     misc_feature    104..960
                     /label=E0840
     misc_feature    84..89
                     /label=critical region for ptsG/SgrS interaction
     misc_feature    85..85
                     /label=C85G mutant of ptsG(wt)
ORIGIN
        1 ataaataaag ggcgcttaga tgccctgtac acggcgaggc tctcccccct tgccacgcgt
       61 gagaacgtaa aaaaagcacc catagtcagg agcactctca attatgcgta aaggagaaga
      121 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      181 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      241 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
      301 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
      361 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      421 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      481 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
      541 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
      601 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
      661 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
      721 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
      781 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
      841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//