##gff-version 3
##sequence-region BBa_K581004 84 960
BBa_K581004	.	point_mutation	87	87	.	+	0	ID=annotation2152638;Name=C87G mutant of ptsG(wt)
BBa_K581004	.	engineered_region	104	960	.	+	0	ID=annotation2152636;Name=E0840
BBa_K581004	.	conserved_region	84	89	.	+	0	ID=annotation2152637;Name=critical region for ptsG/SgrS interaction
>BBa_K581004
ataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcac
ccatactgaggagcactctcaattatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt
gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta
tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta
tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac
acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt
acacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata