LOCUS       BBa_K581008              460 bp    DNA     linear       04-Jul-2024
DEFINITION  RBS+ccdB+Termintor
ACCESSION   BBa_K581008
VERSION     BBa_K581008.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     misc_feature    18..20
                     /label=start
     misc_feature    321..323
                     /label=stop
     CDS             18..320
                     /label=cds
     CDS             18..323
                     /label=BBa_K145151
     stem_loop       343..386
                     /label=stem_loop
     misc_feature    332..411
                     /label=BBa_B0010
     terminator      332..411
                     /label=BBa_B0010
     stem_loop       427..446
                     /label=T7 TE
     misc_feature    453..453
                     /label=stop
     polyA_site      447..460
                     /label=polya
     misc_feature    420..460
                     /label=BBa_B0012
     terminator      420..460
                     /label=BBa_B0012
     terminator      332..460
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga ctactagatg cagtttaagg tttacaccta taaaagagag agccgttatc
       61 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
      121 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
      181 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
      241 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
      301 acctgatgtt ctggggaata taatactaga gccaggcatc aaataaaacg aaaggctcag
      361 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      421 cacactggct caccttcggg tgggcctttc tgcgtttata
//