LOCUS BBa_K581010 4028 bp DNA linear 27-Dec-2024 DEFINITION promoter(BBa_J23106)-RBS-luxU-RBS-cqsS-RBS-luxO ACCESSION BBa_K581010 VERSION BBa_K581010.1 FEATURES Location/Qualifiers misc_feature 1650..1650 /label=A->G to remove EcoR1 site promoter 1..35 /label=J23106 RBS 44..55 /label=B0034 CDS 62..403 /label=luxU RBS 409..421 /label=B0034 CDS 430..2490 /label=cqsS misc_feature 1089..1089 /label=A->G to remove PstI site RBS 2497..2508 /label=B0034 CDS 2515..4028 /label=luxO misc_feature 3234..3234 /label=G->A to remove Pst1 site ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgagagaa tggatcaacc aaagcaaaat cgacttactg gcgaaggaga tcggtgaaga 121 aaacgtgccg atcttggtca acatttttct tggtgaactc aatgattacc aaagtaagtt 181 agtcagtgat acggtcgcgg acaaactcgg gtatctcaag gaaatcagcc atgcgctgaa 241 aagcagtgcg gccagttttg gggctgatcg cttgtgcgcc aaagcggtgg agttagatag 301 tcgcgccaaa tctggcgaga tgatggacat ctcgctagag gttgaacata tgcttgagtt 361 actgaagcag acgcatcaat gctactccga tttagttcat taaggatcca aagaggagaa 421 atactagagg tgatagtgag catggatgta ataaagagag tgtatcaata cgcagaacct 481 aatctgtcct tagtcggatg gatgggcatg cttggctttc ctgcctacta cttcatctgg 541 gaatattggt ttccgcaatc ttacgaaaat ttagggctgc gttgtgcggc agcggtgctg 601 ttcggtgggc tggtgtttcg tgattcgatg cctaagaaat ggcagcggta tatgccgggc 661 tattttttat tcaccatagg tttctgctta ccctttttct tcgcttttat gatgttaatg 721 aatgactggt cgacaatttg ggccatgtcg tttatggcgt ccatttttct gcatattctc 781 ttagtacacg atacgagagt gatggcgtta caagcacttt tctctgtgtt ggtggcctat 841 cttgcggtat atggtttgac ggattttcac ccgacgactt tgattgaatg gcaatacata 901 ccgatctttt tgtttactta tgtatttggg aatttatgtt ttttccgtaa ccaaatctct 961 catgagacca aagtgtcgat tgccaaaacg ttcggagcgg ggattgccca tgaaatgcgt 1021 aatccactca gcgctttgaa aacgtcgatt gatgttgtgc gaaccatgat ccctaaaccg 1081 caaactgcgg ctcatacgga ttattccttg gatgcgcaag agctggatct gctgcatcaa 1141 attttgaatg aagctgatga tgtcatctac tctggcaata acgcgattga tttgctgctc 1201 acctcgattg atgaaaaccg tgtctctccg gcgagtttta aaaaacactc agtggtagat 1261 gtgattgaaa aagcagtgaa aacttttccg tataaaaatg cagcggacca gcactcggta 1321 gagcttgaag tgcatcagcc gtttgacttt tttggcagtg atactctact gacttatgcg 1381 ctatttaacc tgttgaaaaa tgccttctat taccaaaaag agcatttctc ggtttgtatt 1441 tccattgaac aaaccagtga gcataacctt attcgagtcc gtgataacgg tgtggggatt 1501 gcgccagaga tgttggaaga cattttccgt gatttctata ccttcggtaa aaatggtagc 1561 tatgggctag gattgccatt ttgccgcaaa gtgatgtccg catttggggg gacgattcgc 1621 tgtgcgtctc aacaaggtca atggacagag ttcgtattga gctttcctcg ttacgattcc 1681 gatacggtta acgagataaa aacagaactg ctgaaaacca aatcgctcat ttatatcggc 1741 tcaaatcaag ctatcgttcg agagttaaat cagctcgcag tagaagatga gtttggtttt 1801 accgctattt ctgcgcagca agcagtaaga cgccaagatt atgagtttga gttcgacctt 1861 attttgctcg accttgatga cgctacggcg cagggagaac tactgccgaa acttgaaggg 1921 acattgagtt ttgcagaagg ctgtatcggc tatgtgtatg atccgggcaa aacgtatgca 1981 gtaaacatta accgttatct gcgtatccaa ccgatcagta ttcactctat tctcagaaag 2041 ccgcggaaaa tcatcgaacg cttgttgttt gagcaagaat ccttatcgat gaatcgtaac 2101 gtgatcccct tgcaaaagag tcggcatgaa cgacgtattt tggtggttga cgataatcaa 2161 tcgatccgaa cttttacggc tattttgctt gagcaacaag gttatgaagt ggtgcaagcc 2221 aacgatggta gtgaagtgct caagcatatg gagagtcaaa acatcgattt agtattgatg 2281 gacattgaaa tgccgaatgt cgggggatta gaagcgacgc gtttaattcg taactctgag 2341 catgagtaca aaaatattcc gatcattggt tacaccggtg ataacagccc caaaaccttg 2401 gctctggtac aaacctctgg catgaatgat ttcattgtga agcctgctga ccgtgatgta 2461 ttgctaaata aagtggcagc ttgggtgtag gagctcaaag aggagaaata ctagatggta 2521 gaagacacgg cgtcggtggc ggcgctgtat cgttcttacc tcacaccgct ggatattgat 2581 atcaatatcg tgggtaccgg acgcgatgcc atcgagagta ttggccgtcg cgagcctgac 2641 ttaatcctgc tggatttacg tttgccagat atgacgggga tggacgtact ctatgcagtg 2701 aaagagaaat caccggatgt gcctatcgtg tttatgaccg ctcatggttc gattgatacg 2761 gcggttgaag ccatgcgtca tggtgcgcaa gactttttga tcaagccgtg tgaagcggac 2821 cgactgcggg tcacagtgaa taatgcgatt cgcaaagcct cgaaactcaa aaacgatgtt 2881 gataataaaa atcaaaacta tcaaggcttt attggtagca gtcaaaccat gcaggcggtg 2941 taccgcacca ttgactctgc ggcgagcagc aaagccagta tttttattac cggcgaaagt 3001 ggtacaggta aagaagtgtg cgcggaagcg attcatgccg cgagcaagcg tggagataag 3061 ccatttatcg ccatcaactg tgcggccatt ccgaaagatc tgattgaaag tgagttgttt 3121 ggtcacgtca aaggggcttt tactggggcg gcgactgagc gtcaaggcgc ggcagaagcg 3181 gctgatgggg gaaccctctt tttggatgaa ttgtgcgaaa tggatctgga tctacagacc 3241 aaacttctgc gctttattca gacagggaca ttccaaaaag tcggctcttc caaaatgaaa 3301 agcgtggatg tgcgttttgt gtgtgcaacg aaccgcgatc cgtggaagga agtgcaagaa 3361 ggacgctttc gtgaagacct gtactaccgc ttgtatgtga tcccgctgca tttaccgcca 3421 ttgcgcgcgc gtggtgatga tgtgatcgag attgcttatt cactgcttgg ctttatgtcc 3481 aaggaagagg gcaaagattt tgtccgtttg tcggctgaag tggtggagcg ttttcgccaa 3541 tacgagtggc cgggcaacgt gcgtcaattg caaaacgttt tgcgcaacgt ggttgtgctc 3601 aatgaaggtc gtgaaatcac cctagatatg ctgcctcctc ctcttaatca aatgtccgcg 3661 ccgatcaatc gggctttacc gcttgcgcat gagaataaag tatccgtgca tgagattttt 3721 ccgctgtgga tgaccgaaaa acaagccatt gaacaagcca tcgaagcgtg tgatggcaac 3781 attccccgtg ccgcaaccta tctggatgtc agcccgtcaa ccatctatcg caagctgcaa 3841 acttggaatg aaaaagtgaa agaaaaagag aaggaacggt aatactagag ccaggcatca 3901 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3961 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 4021 actagtag //