LOCUS       BBa_K581015             3288 bp    DNA     linear       05-Nov-2024
DEFINITION  pBAD-supD-plux_inv-T7ptag
ACCESSION   BBa_K581015
VERSION     BBa_K581015.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    139..274
                     /label=BBa_K228001
     stem_loop       294..337
                     /label=stem_loop
     misc_feature    283..362
                     /label=BBa_B0010
     terminator      283..362
                     /label=BBa_B0010
     stem_loop       378..397
                     /label=T7 TE
     misc_feature    404..404
                     /label=stop
     polyA_site      398..411
                     /label=polya
     misc_feature    371..411
                     /label=BBa_B0012
     terminator      371..411
                     /label=BBa_B0012
     terminator      283..411
                     /label=BBa_B0015
     promoter        420..425
                     /label=-35
     promoter        444..449
                     /label=-10
     misc_feature    425..444
                     /label=Lux Box
     promoter        420..449
                     /label=BBa_R0061
     RBS             458..472
                     /label=RBS-1\Strong
     RBS             465..469
                     /label=RBS
     misc_feature    458..472
                     /label=BBa_B0030
     RBS             458..472
                     /label=BBa_B0030
     CDS             479..3151
                     /label=cds
     CDS             479..3151
                     /label=BBa_K228000
     stem_loop       3171..3214
                     /label=stem_loop
     misc_feature    3160..3239
                     /label=BBa_B0010
     terminator      3160..3239
                     /label=BBa_B0010
     stem_loop       3255..3274
                     /label=T7 TE
     misc_feature    3281..3281
                     /label=stop
     polyA_site      3275..3288
                     /label=polya
     misc_feature    3248..3288
                     /label=BBa_B0012
     terminator      3248..3288
                     /label=BBa_B0012
     terminator      3160..3288
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagca attcggagag atgccggagc ggctgaacgg accggtctct
      181 aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc gccactacag
      241 atccttagcg aaagctaagg atttttttta agcttactag agccaggcat caaataaaac
      301 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      361 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt
      421 tgacacctgt aggatcgtac aggtataatt actagagatt aaagaggaga aatactagat
      481 gaccatgatt accgtgcact agaataccat taacattgct aagaacgact tctctgacat
      541 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg
      601 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat
      661 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat
      721 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc
      781 taagcgcggc aagcgcccga cagccttcca gttcctgtag gaaatcaagc cggaagccgt
      841 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt
      901 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat
      961 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt
     1021 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg
     1081 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt
     1141 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc
     1201 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat
     1261 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc
     1321 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct
     1381 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc
     1441 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt
     1501 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc
     1561 gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct
     1621 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg
     1681 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat
     1741 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc
     1801 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa
     1861 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc
     1921 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa
     1981 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt
     2041 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct
     2101 ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt
     2161 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt
     2221 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt
     2281 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa
     2341 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt
     2401 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac
     2461 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc
     2521 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga
     2581 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa
     2641 gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc
     2701 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca
     2761 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca
     2821 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac
     2881 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt
     2941 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga
     3001 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca
     3061 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga
     3121 catcttagag tcggacttcg cgttcgcata atactagagc caggcatcaa ataaaacgaa
     3181 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     3241 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//