##gff-version 3
##sequence-region BBa_K581015 1 3288
BBa_K581015	.	CDS	479	3151	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K581015	.	CDS	479	3151	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K581015	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K581015	.	promoter	420	449	.	+	0	ID=BBa_R0061;Name=BBa_R0061
BBa_K581015	.	non_covalent_binding_site	425	444	.	+	0	ID=annotation305877;Name=Lux Box;Parent=BBa_R0061
BBa_K581015	.	promoter	420	425	.	+	0	ID=annotation305863;Name=-35;Parent=BBa_R0061
BBa_K581015	.	promoter	444	449	.	+	0	ID=annotation305864;Name=-10;Parent=BBa_R0061
BBa_K581015	.	terminator	283	411	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K581015	.	terminator	283	362	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K581015	.	stem_loop	294	337	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K581015	.	engineered_region	283	362	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K581015	.	terminator	371	411	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K581015	.	engineered_region	371	411	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K581015	.	stop_codon	404	404	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K581015	.	stem_loop	378	397	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K581015	.	polyA_site	398	411	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K581015	.	ribosome_entry_site	458	472	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K581015	.	engineered_region	458	472	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K581015	.	ribosome_entry_site	465	469	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K581015	.	ribosome_entry_site	458	472	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K581015	.	mature_transcript_region	139	274	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K581015	.	terminator	3160	3288	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K581015	.	terminator	3160	3239	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K581015	.	stem_loop	3171	3214	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K581015	.	engineered_region	3160	3239	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K581015	.	terminator	3248	3288	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K581015	.	engineered_region	3248	3288	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K581015	.	stop_codon	3281	3281	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K581015	.	stem_loop	3255	3274	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K581015	.	polyA_site	3275	3288	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K581015
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccgga
gcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccacta
cagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagttgacacctgtaggatcgtacaggtataattactagagattaaagaggagaaata
ctagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgct
atcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacg
agatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaa
gcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggc
aagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctgg
cttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcg
cttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcac
gtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtctt
cgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagctt
acaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgca
acccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcatta
ctggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgcta
cgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtc
ctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcc
cgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaa
ggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggcc
atctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatga
ccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaa
ctgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgc
gctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacg
ctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagca
cttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctac
gggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtga
ccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacgg
tgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtg
ctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggataca
tggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgc
taagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaact
cctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttcc
gcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactt
tgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgca
ctgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgaca
catatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaat
gccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata