LOCUS BBa_K581017 3681 bp DNA linear 09-May-2024 DEFINITION araC-pBAD-supD-nahR-psal-supD-pT7-rbs-gfp ACCESSION BBa_K581017 VERSION BBa_K581017.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 misc_feature 1288..1423 /label=BBa_K228001 stem_loop 1443..1486 /label=stem_loop misc_feature 1432..1511 /label=BBa_B0010 terminator 1432..1511 /label=BBa_B0010 stem_loop 1527..1546 /label=T7 TE misc_feature 1553..1553 /label=stop polyA_site 1547..1560 /label=polya misc_feature 1520..1560 /label=BBa_B0012 terminator 1520..1560 /label=BBa_B0012 terminator 1432..1560 /label=BBa_B0015 CDS 1569..2471 /label=NahR promoter 2472..2561 /label=Pr promoter promoter 2550..2618 /label=pSal promoter 1569..2620 /label=BBa_K228004 misc_feature 2629..2764 /label=BBa_K228001 promoter 2773..2795 /label=BBa_I719005 RBS 2804..2818 /label=RBS-1\Strong RBS 2811..2815 /label=RBS misc_feature 2804..2818 /label=BBa_B0030 RBS 2804..2818 /label=BBa_B0030 CDS 2825..3544 /label=GFP protein CDS 2825..3544 /label=BBa_E0040 stem_loop 3564..3607 /label=stem_loop misc_feature 3553..3632 /label=BBa_B0010 terminator 3553..3632 /label=BBa_B0010 stem_loop 3648..3667 /label=T7 TE misc_feature 3674..3674 /label=stop polyA_site 3668..3681 /label=polya misc_feature 3641..3681 /label=BBa_B0012 terminator 3641..3681 /label=BBa_B0012 misc_feature 2804..3681 /label=BBa_E0840 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga 1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg 1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gccaggcatc 1441 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1501 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1561 tactagagtc aatccgtaaa caggtcaaac atcagttgcc gcaaccaaat attggctagg 1621 tccttgtggt acttcgcatg ccagaacatg ttgatggcta tttcaggcaa gacgactggg 1681 tgcggcaagg cgcttaggcc gaagggctcc acgcagcagt cggctaaacg tatcggcaca 1741 gtggcgagca gatcggtgcg ctggaggatg tggccaacgg cggcgaagtg cggcacttcc 1801 agacggatgt cgcgccggat gccgacccgt gtcatgtacg tgtccacctc gccgtggccg 1861 gtgccagcgg cgatgacacg cacgtggccg taggaacaga agcgctccag agtcaggggt 1921 tcgcgggtga ctggatggtc cttgcgacat aggcacacgt agtgattctg gagcagccgg 1981 cgctgaaaga agccagtttg cagattggga agcaggccca cggccaagtc cacggttccg 2041 ttctgcaagg cctgcatcag gctcatcgaa ctgtcgcgca ccgtactgat cacgcaattg 2101 ggggcctggt gagccagcac atccatcagc cgcggcatga agtagatctc gccaatgtcg 2161 gtcatggcca gggtgaaggt acgctcgctg gtcagcggat cgaagctttc atggtgctgt 2221 agggcgttgc gcagtgcgtg catggccgaa gtgacgggct cggccagatg cgcggcatag 2281 ggtgtgggtt ccattccctg atgtgtgcgc acgaagagtg ggtcctgtag cgaggtgcgc 2341 aggcgtttca gcgcattgct cacggcaggc tgggtcaggc ccaggttctc cgcagtgata 2401 gagacgcgtc tgtcgaccag caactggttg aacaccacca gcaggtttaa atccaggtca 2461 cgcagttcca tggggcctcg cttgggttat tgctggtgcc cggccgggcg caatattcat 2521 gttgatgatt tattatatat cgagtggtgt atttatcaat attgtttgct ccgttatcgt 2581 tattaacaag tcatcaataa agccatcacg agtaccatag tactagagca attcggagag 2641 atgccggagc ggctgaacgg accggtctct aaaaccggag taggggcaac tctaccgggg 2701 gttcaaatcc ccctctctcc gccactacag atccttagcg aaagctaagg atttttttta 2761 agcttactag agtaatacga ctcactatag ggagatacta gagattaaag aggagaaata 2821 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga 2881 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata 2941 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac 3001 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa 3061 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt 3121 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct 3181 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca 3241 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa 3301 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc 3361 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca 3421 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt 3481 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata 3541 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3601 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 3661 gtgggccttt ctgcgtttat a //