##gff-version 3
##sequence-region BBa_K581017 1 3681
BBa_K581017	.	terminator	1101	1141	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K581017	.	stop_codon	1134	1134	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K581017	.	polyA_site	1128	1141	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K581017	.	stem_loop	1108	1127	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K581017	.	engineered_region	1101	1141	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K581017	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K581017	.	engineered_region	1	1141	.	+	0	ID=BBa_K228008;Name=BBa_K228008
BBa_K581017	.	polyA_site	1128	1141	.	+	0	ID=annotation2020013;Name=polya;Parent=BBa_K228008
BBa_K581017	.	promoter	27	32	.	+	0	ID=annotation2020001;Name=-10;Parent=BBa_K228008
BBa_K581017	.	engineered_region	47	58	.	+	0	ID=annotation2020003;Name=BBa_B0034;Parent=BBa_K228008
BBa_K581017	.	engineered_region	47	1141	.	+	0	ID=annotation2020015;Name=BBa_K228006;Parent=BBa_K228008
BBa_K581017	.	conserved_region	51	54	.	+	0	ID=annotation2020004;Name=conserved;Parent=BBa_K228008
BBa_K581017	.	engineered_region	1013	1092	.	+	0	ID=annotation2020009;Name=BBa_B0010;Parent=BBa_K228008
BBa_K581017	.	stop_codon	1134	1134	.	+	0	ID=annotation2020014;Name=stop;Parent=BBa_K228008
BBa_K581017	.	engineered_region	1150	1279	.	+	0	ID=annotation2020016;Name=BBa_I13453;Parent=BBa_K228008
BBa_K581017	.	stem_loop	1024	1067	.	+	0	ID=annotation2020010;Name=stem_loop;Parent=BBa_K228008
BBa_K581017	.	tag	941	973	.	+	0	ID=annotation2020007;Name=LVA;Parent=BBa_K228008
BBa_K581017	.	stem_loop	1108	1127	.	+	0	ID=annotation2020012;Name=T7 TE;Parent=BBa_K228008
BBa_K581017	.	operator	1	38	.	+	0	ID=annotation2019999;Name=P(Cat);Parent=BBa_K228008
BBa_K581017	.	engineered_region	1101	1141	.	+	0	ID=annotation2020011;Name=BBa_B0012;Parent=BBa_K228008
BBa_K581017	.	engineered_region	65	979	.	+	0	ID=annotation2020005;Name=BBa_C0080;Parent=BBa_K228008
BBa_K581017	.	promoter	4	9	.	+	0	ID=annotation2020000;Name=-35;Parent=BBa_K228008
BBa_K581017	.	engineered_region	1	38	.	+	0	ID=annotation2020002;Name=BBa_I14033;Parent=BBa_K228008
BBa_K581017	.	CDS	65	940	.	+	0	ID=annotation2020006;Name=araC;Parent=BBa_K228008
BBa_K581017	.	engineered_region	980	1004	.	+	0	ID=annotation2020008;Name=barcode;Parent=BBa_K228008
BBa_K581017	.	terminator	1432	1560	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K581017	.	terminator	1432	1511	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K581017	.	engineered_region	1432	1511	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K581017	.	stem_loop	1443	1486	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K581017	.	terminator	1520	1560	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K581017	.	stop_codon	1553	1553	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K581017	.	polyA_site	1547	1560	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K581017	.	stem_loop	1527	1546	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K581017	.	engineered_region	1520	1560	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K581017	.	promoter	1150	1279	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K581017	.	mature_transcript_region	1288	1423	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K581017	.	engineered_region	2804	3681	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K581017	.	ribosome_entry_site	2804	2818	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K581017	.	ribosome_entry_site	2811	2815	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K581017	.	engineered_region	2804	2818	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K581017	.	ribosome_entry_site	2804	2818	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K581017	.	terminator	3641	3681	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K581017	.	stop_codon	3674	3674	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K581017	.	polyA_site	3668	3681	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K581017	.	stem_loop	3648	3667	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K581017	.	engineered_region	3641	3681	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K581017	.	CDS	2825	3544	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K581017	.	CDS	2825	3544	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K581017	.	terminator	3553	3632	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K581017	.	engineered_region	3553	3632	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K581017	.	stem_loop	3564	3607	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K581017	.	mature_transcript_region	2629	2764	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K581017	.	promoter	1569	2620	.	+	0	ID=BBa_K228004;Name=BBa_K228004
BBa_K581017	.	promoter	2550	2618	.	+	0	ID=annotation2075287;Name=pSal;Parent=BBa_K228004
BBa_K581017	.	CDS	1569	2471	.	+	0	ID=annotation2075285;Name=NahR;Parent=BBa_K228004
BBa_K581017	.	promoter	2472	2561	.	+	0	ID=annotation2075286;Name=Pr promoter;Parent=BBa_K228004
BBa_K581017	.	promoter	2773	2795	.	+	0	ID=BBa_I719005;Name=BBa_I719005
BBa_K581017	.	terminator	1013	1092	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K581017	.	engineered_region	1013	1092	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K581017	.	stem_loop	1024	1067	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K581017
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatc
agtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttc
acaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaa
ccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcct
cgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctg
tgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatcc
atcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagct
ccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatc
cgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaa
acccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatat
cacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacc
tttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgg
gcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgt
ttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatg
ccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccg
tttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtagg
ggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagc
ttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcaatccgtaaa
caggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggct
atttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggca
cagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccg
gatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaa
cagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagca
gccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctg
catcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgc
ggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcat
ggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttc
cattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggc
tgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggttta
aatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatga
tttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatc
acgagtaccatagtactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtagggg
caactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagctt
actagagtaatacgactcactatagggagatactagagattaaagaggagaaatactagatgcgtaaaggagaagaact
tttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggt
gaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacac
ttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagag
tgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtc
aagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggac
acaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaactt
caaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggc
cctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccaca
tggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata