BBa_K581017
type: ComponentDefinition
persistent identity: BBa K581017
display id: BBa_K581017
version: 1
was generated by: iGEM to SBOL conversion
description: araC-pBAD-supD-nahR-psal-supD-pT7-rbs-gfp
created: 2011-09-27T11:00:00Z
modified: 2015-05-08T01:12:46Z
mutable provenance: Assembled from parts in the Registry.
owned by: james
owned by: myers
mutable description: It is a subpart of our XOR Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _752_
m user id: 0
owner id: 8716
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: It is a subpart of our XOR Gate.
star: false
creator: Handuo SHI
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K228001
component: BBa_I13453
component: BBa_K228008
component: BBa_E0840
component: BBa_I719005
component: BBa_K228004
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K228008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I13453
sequence annotation: BBa_K228001
sequence annotation: BBa_B0015
sequence annotation: BBa_K228004
sequence annotation: BBa_I719005
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K581017_sequence/1

BBa_B0015
type: Component
persistent identity: component2298418
display id: component2298418
version: 1
top level: BBa_K581017
definition: BBa_B0015
access: public

BBa_K228001
type: Component
persistent identity: component2298411
display id: component2298411
version: 1
top level: BBa_K581017
definition: BBa_K228001
access: public

BBa_I13453
type: Component
persistent identity: component2298410
display id: component2298410
version: 1
top level: BBa_K581017
definition: BBa_I13453
access: public

BBa_K228008
type: Component
persistent identity: component2298409
display id: component2298409
version: 1
top level: BBa_K581017
definition: BBa_K228008
access: public

BBa_E0840
type: Component
persistent identity: component2298435
display id: component2298435
version: 1
top level: BBa_K581017
definition: GFP genera
access: public

BBa_I719005
type: Component
persistent identity: component2298424
display id: component2298424
version: 1
top level: BBa_K581017
definition: pT7
access: public

BBa_K228001
type: Component
persistent identity: component2298423
display id: component2298423
version: 1
top level: BBa_K581017
definition: BBa_K228001
access: public

BBa_I13453
type: Component
persistent identity: component2298408
display id: component2298408
version: 1
top level: BBa_K581017
definition: BBa_I13453
access: public

BBa_K228004
type: Component
persistent identity: component2298422
display id: component2298422
version: 1
top level: BBa_K581017
definition: BBa_K228004
access: public

BBa_B0012
type: Component
persistent identity: component2298404
display id: component2298404
version: 1
top level: BBa_K581017
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2298402
display id: component2298402
version: 1
top level: BBa_K581017
definition: BBa_B0010
access: public

BBa_K228008
type: SequenceAnnotation
persistent identity: annotation2298409
display id: annotation2298409
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298409/range2298409/1
component: BBa_K228008

https://synbiohub.org/public/igem/BBa_K581017/annotation2298409/range2298409/1
type: Range
persistent identity: range2298409
display id: range2298409
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 1
end: 1141
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2298402
display id: annotation2298402
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298402/range2298402/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K581017/annotation2298402/range2298402/1
type: Range
persistent identity: range2298402
display id: range2298402
version: 1
top level: BBa_K581017
direction: direction/forward
start: 1013
end: 1092
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2298404
display id: annotation2298404
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298404/range2298404/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K581017/annotation2298404/range2298404/1
type: Range
persistent identity: range2298404
display id: range2298404
version: 1
top level: BBa_K581017
direction: direction/forward
start: 1101
end: 1141
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2298408
display id: annotation2298408
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298408/range2298408/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K581017/annotation2298408/range2298408/1
type: Range
persistent identity: range2298408
display id: range2298408
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2298410
display id: annotation2298410
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298410/range2298410/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K581017/annotation2298410/range2298410/1
type: Range
persistent identity: range2298410
display id: range2298410
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2298411
display id: annotation2298411
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298411/range2298411/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K581017/annotation2298411/range2298411/1
type: Range
persistent identity: range2298411
display id: range2298411
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 1288
end: 1423
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2298418
display id: annotation2298418
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298418/range2298418/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K581017/annotation2298418/range2298418/1
type: Range
persistent identity: range2298418
display id: range2298418
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 1432
end: 1560
orientation: inline

BBa_K228004
type: SequenceAnnotation
persistent identity: annotation2298422
display id: annotation2298422
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298422/range2298422/1
component: BBa_K228004

https://synbiohub.org/public/igem/BBa_K581017/annotation2298422/range2298422/1
type: Range
persistent identity: range2298422
display id: range2298422
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 1569
end: 2620
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2298423
display id: annotation2298423
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298423/range2298423/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K581017/annotation2298423/range2298423/1
type: Range
persistent identity: range2298423
display id: range2298423
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 2629
end: 2764
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2298424
display id: annotation2298424
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298424/range2298424/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K581017/annotation2298424/range2298424/1
type: Range
persistent identity: range2298424
display id: range2298424
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 2773
end: 2795
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2298435
display id: annotation2298435
version: 1
top level: BBa_K581017
location: https://synbiohub.org/public/igem/BBa_K581017/annotation2298435/range2298435/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K581017/annotation2298435/range2298435/1
type: Range
persistent identity: range2298435
display id: range2298435
version: 1
top level: BBa_K581017
direction: direction/reverse
start: 2804
end: 3681
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_K228008
type: ComponentDefinition
persistent identity: BBa K228008
display id: BBa_K228008
version: 1
was generated by: iGEM to SBOL conversion
description: AraC regulatory protein
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part could be connected to a protein coding sequence so that the expression could be induced by L-arabinose
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_I14033
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: BBa_K228006
sequence annotation: conserved
sequence annotation: araC
sequence annotation: BBa_C0080
sequence annotation: LVA
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228008_sequence/1

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2020002
display id: annotation2020002
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
type: Range
persistent identity: range2020002
display id: range2020002
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1
end: 38
orientation: inline

P(Cat)
type: SequenceAnnotation
persistent identity: annotation2019999
display id: annotation2019999
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
type: Range
persistent identity: range2019999
display id: range2019999
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2020000
display id: annotation2020000
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
type: Range
persistent identity: range2020000
display id: range2020000
version: 1
top level: BBa_K228008
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2020001
display id: annotation2020001
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
type: Range
persistent identity: range2020001
display id: range2020001
version: 1
top level: BBa_K228008
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2020003
display id: annotation2020003
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
type: Range
persistent identity: range2020003
display id: range2020003
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 58
orientation: inline

BBa_K228006
type: SequenceAnnotation
persistent identity: annotation2020015
display id: annotation2020015
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
type: Range
persistent identity: range2020015
display id: range2020015
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 1141
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2020004
display id: annotation2020004
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
type: Range
persistent identity: range2020004
display id: range2020004
version: 1
top level: BBa_K228008
direction: direction/forward
start: 51
end: 54
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2020006
display id: annotation2020006
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
type: Range
persistent identity: range2020006
display id: range2020006
version: 1
top level: BBa_K228008
direction: direction/forward
start: 65
end: 940
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2020005
display id: annotation2020005
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
type: Range
persistent identity: range2020005
display id: range2020005
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 65
end: 979
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2020007
display id: annotation2020007
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
type: Range
persistent identity: range2020007
display id: range2020007
version: 1
top level: BBa_K228008
direction: direction/forward
start: 941
end: 973
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2020008
display id: annotation2020008
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
type: Range
persistent identity: range2020008
display id: range2020008
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 980
end: 1004
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2020009
display id: annotation2020009
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
type: Range
persistent identity: range2020009
display id: range2020009
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1013
end: 1092
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2020010
display id: annotation2020010
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
type: Range
persistent identity: range2020010
display id: range2020010
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1024
end: 1067
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020011
display id: annotation2020011
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
type: Range
persistent identity: range2020011
display id: range2020011
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1101
end: 1141
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2020012
display id: annotation2020012
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
type: Range
persistent identity: range2020012
display id: range2020012
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1108
end: 1127
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2020013
display id: annotation2020013
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
type: Range
persistent identity: range2020013
display id: range2020013
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1128
end: 1141
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2020014
display id: annotation2020014
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
type: Range
persistent identity: range2020014
display id: range2020014
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1134
end: 1134
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2020016
display id: annotation2020016
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
type: Range
persistent identity: range2020016
display id: range2020016
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_K228004
type: ComponentDefinition
persistent identity: BBa K228004
display id: BBa_K228004
version: 1
was generated by: iGEM to SBOL conversion
description: NahR( reverse) - salicylate promoter
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: It is constrcuted by PCR from the plasmid kindly provided by Voigt Lab in UCSF.
owned by: james
owned by: myers
mutable description: This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.
star: false
creator: Lin Min
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: NahR
sequence annotation: Pr promoter
sequence annotation: pSal
sequence: https://synbiohub.org/public/igem/BBa_K228004_sequence/1

NahR
type: SequenceAnnotation
persistent identity: annotation2075285
display id: annotation2075285
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
type: Range
persistent identity: range2075285
display id: range2075285
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 1
end: 903
orientation: inline

Pr promoter
type: SequenceAnnotation
persistent identity: annotation2075286
display id: annotation2075286
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
type: Range
persistent identity: range2075286
display id: range2075286
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 904
end: 993
orientation: inline

pSal
type: SequenceAnnotation
persistent identity: annotation2075287
display id: annotation2075287
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
type: Range
persistent identity: range2075287
display id: range2075287
version: 1
top level: BBa_K228004
direction: direction/forward
start: 982
end: 1050
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0030.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0030
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
definition: BBa_B0030
access: public

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
definition: BBa_B0012
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
top level: GFP genera
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
top level: GFP genera
direction: direction/forward
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
top level: GFP genera
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
top level: GFP genera
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K581017_sequence/1
type: Sequence
persistent identity: BBa K581017 sequence
display id: BBa_K581017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228008_sequence/1
type: Sequence
persistent identity: BBa K228008 sequence
display id: BBa_K228008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228004_sequence/1
type: Sequence
persistent identity: BBa K228004 sequence
display id: BBa_K228004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0840_sequence/1
type: Sequence
persistent identity: BBa E0840 sequence
display id: BBa_E0840_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z